Abstract: The invention is a method for estimating a representative volume of particles of interest (10 i) immersed in a sample, the sample extending in at least one plane, referred to as the sample plane (P 10), the sample comprising a sphering agent, capable of modifying the shape of the particles, the method comprising the following steps: a) illuminating the sample by means of a light source (11), the light source emitting an incident light wave (12) propagating towards the sample (10) along a propagation axis (Z); b) acquiring, by means of an image sensor (16), an image (I 0) of the sample (10), formed in a detection plane (P 0), the sample being arranged between the light source (11) and the image sensor (16), each image being representative of a light wave (14) referred to as an exposure light wave, to which the image sensor (16) is exposed under the effect of illumination; c) using the image of the sample (I 0), acquired during step b), and a holographic propagation operator, to calculate a complex expression (

Abstract: The invention relates to a method for counting particles, particularly blood cells, in a sample, using a lensless optical imaging device. The sample is arranged between a light source and an image sensor. The sample is illuminated by a light source and an image is acquired by the image sensor, said image sensor being exposed to a light wave called an exposition wave. A digital propagation operator is applied to the acquired image so as to obtain a complex amplitude of the exposition wave according to a surface facing the image sensor. An image, called a reconstructed image, is formed from the modulus and/or the phase of said complex amplitude, on which image the particles to be counted appear in the form of regions of interest. The method then comprises a step of selecting the regions of interest corresponding to the particles to be counted.

Abstract: The invention relates to a method for counting particles, particularly blood cells, in a sample, using a lensless optical imaging device. The sample is arranged between a light source and an image sensor. The sample is illuminated by a light source and an image is acquired by the image sensor, said image sensor being exposed to a light wave called an exposition wave. A digital propagation operator is applied to the acquired image so as to obtain a complex amplitude of the exposition wave according to a surface facing the image sensor. An image, called a reconstructed image, is formed from the modulus and/or the phase of said complex amplitude, on which image the particles to be counted appear in the form of regions of interest. The method then comprises a step of selecting the regions of interest corresponding to the particles to be counted.

Abstract: The invention is a method for estimating a representative volume of particles of interest (10 i) immersed in a sample, the sample extending in at least one plane, referred to as the sample plane (P 10), the sample comprising a sphering agent, capable of modifying the shape of the particles, the method comprising the following steps: a) illuminating the sample by means of a light source (11), the light source emitting an incident light wave (12) propagating towards the sample (10) along a propagation axis (Z); b) acquiring, by means of an image sensor (16), an image (I 0) of the sample (10), formed in a detection plane (P 0), the sample being arranged between the light source (11) and the image sensor (16), each image being representative of a light wave (14) referred to as an exposure light wave, to which the image sensor (16) is exposed under the effect of illumination; c) using the image of the sample (I 0), acquired during step b), and a holographic propagation operator, to calculate a complex expression (