Abstract: There is provided an information provision method for providing support information for supporting a judgement based on information obtained from a tissue section. The method includes: obtaining a digital bright-field image of a tissue section stained to be observable in a bright field; creating an analysis score by obtaining, combining, and scoring multiple kinds of information on the bright-field image; and presenting the analysis score as the support information.

Abstract: The present invention relates to a system for acquiring information on a target protein in a sample by detecting the target protein expressed in an immune cell such as a macrophage with high accuracy, the information acquisition system including a step (A) of staining a first immune cell protein, a step (B) of staining a second immune cell protein, a step (C) of staining the target protein, and a step (D) of measuring, after the steps (A) to (C), a signal derived from the target protein.

Abstract: The present invention provides a method of obtaining information for the diagnosis or treatment, mainly of cancer or an immune system-related disease, the method including the step of quantifying the expression level of a cancer-associated protein or a nucleic acid in a tumor cell; and/or the step of quantifying the expression level of a protein or a nucleic acid in an immune cell, using a specimen derived from tumor tissue of a human or a non-human.

Abstract: The present invention provides a method for evaluating a surface state of particles for judging suitability of colored particles used for staining a biological material. The method includes: a dispersion step of dispersing colored particles in a two-component dispersion medium containing two types of dispersion media having different polarities to form an interface; an indicator acquisition step of acquiring an indicator indicating the amount of particles contained in one of the two types of dispersion media; and a judgement step of judging, on the basis of the indicator, suitability of a surface state of the colored particles for staining a biological material.