Patents by Inventor Etsuko Kiyokawa

Etsuko Kiyokawa has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Linker for unimolecular FRET biosensor based on principle of fluorescence resonance energy transfer
    Patent number: 9103790
    Abstract: A linker for a unimolecular FRET biosensor based on a principle of fluorescence resonance energy transfer, the linker including: a polypeptide containing 52 to 400 amino acids residues, wherein at least 45% of a total number of the amino acid residues are glycine, alanine, or both thereof, and at least 10% of the total number of the amino acid residues are alanine.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: August 11, 2015
    Assignee: KYOTO UNIVERSITY
    Inventors: Michiyuki Matsuda, Naoki Komatsu, Kazuhiro Aoki, Yuji Kamioka, Hiroko Yukinaga, Yoshie Inaoka, Atsuro Sakurai, Etsuko Kiyokawa, Kenta Sumiyama
  • Sphingomyelin detecting probe
    Patent number: 7217793
    Abstract: Proteins useful as sphingomyelin detecting probes, which specifically recognize sphingomyelin and have low cytotoxicity. The proteins of the present invention may be obtained by deletion of between about 110 to 170 amino acid residues from the N terminal or C terminal of earthworm toxin Lysenin 1. Kits for detecting sphingomyelin are also disclosed.
    Type: Grant
    Filed: September 13, 2005
    Date of Patent: May 15, 2007
    Assignee: Riken
    Inventors: Toshihide Kobayashi, Etsuko Kiyokawa, Akiko Hasegawa
  • Sphingomyelin detecting probe
    Patent number: 6995236
    Abstract: An object of the present invention is to provide a protein useful as a sphingomyelin detecting probe, which specifically recognizes sphingomyelin and has low cytotoxicity. The present invention provides a protein which has an amino acid sequence having, as the amino acid sequence from the 1st to the 48th amino acid, the amino acid sequence from the 1st to the 48th amino acid in Lysenin 1, and as the amino acid sequence from the 49th to the 298th amino acid, the amino acid sequence from the 51st to the 300th amino acid in Lysenin 3; and a protein which is obtained by deleting N terminal and/or C terminal from earthworm toxins Lysenin 1 or 3, and which specifically recognizes sphingomyelin.
    Type: Grant
    Filed: May 6, 2002
    Date of Patent: February 7, 2006
    Assignee: Riken
    Inventors: Toshihide Kobayashi, Etsuko Kiyokawa, Akiko Hasegawa
  • Sphingomyelin detecting probe
    Publication number: 20060014217
    Abstract: An object of the present invention is to provide a protein useful as a sphingomyelin detecting probe, which specifically recognizes sphingomyelin and has low cytotoxicity. The present invention provides a protein which has an amino acid sequence having, as the amino acid sequence from the 1st to the 48th amino acid, the amino acid sequence from the 1st to the 48th amino acid in Lysenin 1, and as the amino acid sequence from the 49th to the 298th amino acid, the amino acid sequence from the 51st to the 300th amino acid in Lysenin 3; and a protein which is obtained by deleting N terminal and/or C terminal from earthworm toxins Lysenin 1 or 3, and which specifically recognizes sphingomyelin.
    Type: Application
    Filed: September 13, 2005
    Publication date: January 19, 2006
    Applicant: RIKEN
    Inventors: Toshihide Kobayashi, Etsuko Kiyokawa, Akiko Hasegawa
  • Sphingomyelin detecting probe
    Publication number: 20020168725
    Abstract: An object of the present invention is to provide a protein useful as a sphingomyelin detecting probe, which specifically recognizes sphingomyelin and has low cytotoxicity. The present invention provides a protein which has an amino acid sequence having, as the amino acid sequence from the 1st to the 48th amino acid, the amino acid sequence from the 1st to the 48th amino acid in Lysenin 1, and as the amino acid sequence from the 49th to the 298th amino acid, the amino acid sequence from the 51st to the 300th amino acid in Lysenin 3; and a protein which is obtained by deleting N terminal and/or C terminal from earthworm toxins Lysenin 1 or 3, and which specifically recognizes sphingomyelin.
    Type: Application
    Filed: May 6, 2002
    Publication date: November 14, 2002
    Applicant: RIKEN
    Inventors: Toshihide Kobayashi, Etsuko Kiyokawa, Akiko Hasegawa