Abstract: The present disclosure relates to sterilization assemblies and related methods and, more particularly, to assemblies and methods for ultraviolet (“UV”) light sterilization of items (e.g., container members and/or packaging, such as packaged containers that house products) for subsequent introduction of the sterilized items to a desired environment (e.g., to an ISO rated cleanroom environment for processing pharmaceutical products; to an isolator environment positioned in a cleanroom for processing pharmaceutical products). The present disclosure provides for improved assemblies/methods for sterilizing items for subsequent introduction of the sterilized items to a desired environment.