Patents by Inventor Eugene G. Spier
Eugene G. Spier has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20200392571Abstract: Methods and systems for ordering assays which detect SNPs or gene expression are provided. The methods use PCR and RT-PCR procedures. Collections of stock assays are assembled using pre- and post-manufacturing quality control procedures and made available to consumers via the Internet. In addition, custom assays are prepared upon order from the consumer and these assays are also prepared using pre- and post-manufacturing quality control procedures. The assays are then delivered to the consumer.Type: ApplicationFiled: June 5, 2020Publication date: December 17, 2020Applicant: APPLIED BIOSYSTEMS, LLCInventors: Ryan T. KOEHLER, Kenneth J. LIVAK, Junko STEVENS, Francisco M. DE LA VEGA, Michael RHODES, Laurent R. BELLON, Dawn MADDEN, Dennis A. GILBERT, Yu N. WANG, Eugene G. SPIER, Xiaoqing YOU, Lily XU, Jeremy HEIL, Emily S. WINN-DEEN, Ivy McMULLEN, Susan EDDINS, Leila G. SMITH
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Patent number: 10689692Abstract: Methods and systems for ordering assays which detect SNPs or gene expression are provided. The methods use PCR and RT-PCR procedures. Collections of stock assays are assembled using pre- and post-manufacturing quality control procedures and made available to consumers via the Internet. In addition, custom assays are prepared upon order from the consumer and these assays are also prepared using pre- and post-manufacturing quality control procedures. The assays are then delivered to the consumer.Type: GrantFiled: September 6, 2016Date of Patent: June 23, 2020Assignee: APPLIED BIOSYSTEMS, LLCInventors: Ryan T. Koehler, Kenneth J. Livak, Junko Stevens, Francisco M. De La Vega, Michael Rhodes, Laurent R. Bellon, Dawn Madden, Dennis A. Gilbert, Yu N. Wang, Eugene G. Spier, Xiaoqing You, Lily Xu, Jeremy Heil, Emily S. Winn-Deen, Ivy McMullen, Susan Eddins, Leila G. Smith
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Publication number: 20170268051Abstract: Methods and systems for ordering assays which detect SNPs or gene expression are provided. The methods use PCR and RT-PCR procedures. Collections of stock assays are assembled using pre- and post-manufacturing quality control procedures and made available to consumers via the Internet. In addition, custom assays are prepared upon order from the consumer and these assays are also prepared using pre- and post-manufacturing quality control procedures. The assays are then delivered to the consumer.Type: ApplicationFiled: September 6, 2016Publication date: September 21, 2017Inventors: Ryan T. Koehler, Kenneth J. Livak, Junko STEVENS, Francisco M. DE LA VEGA, Michael RHODES, Laurent R. BELLON, Yu N. WANG, Xiaoqing YOU, Michael W. HUNKAPILLER, Janet S. ZIEGLE, Julie WILLIAMS, Dawn MADDEN, Dennis A. GILBERT, Charles R. SCAFE, Hadar I. ISAAC, Eugene G. SPIER, Lily XU, Leila G. SMITH, Jeremy HEIL, Stephen GLANOWSKI, John SCOTT, Emily S. WINN-DEEN, Ivy MCMULLEN, Lini WU, Harold Carey GIRE, Susan EDDINS
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Patent number: 9464320Abstract: Methods and systems for ordering assays which detect SNPs or gene expression are provided. The methods use PCR and RT-PCR procedures. Collections of stock assays are assembled using pre- and post-manufacturing quality control procedures and made available to consumers via the Internet. In addition, custom assays are prepared upon order from the consumer and these assays are also prepared using pre- and post-manufacturing quality control procedures. The assays are then delivered to the consumer.Type: GrantFiled: April 27, 2012Date of Patent: October 11, 2016Assignee: Applied Biosystems, LLCInventors: Ryan T. Koehler, Kenneth J. Livak, Junko Stevens, Francisco M. De La Vega, Michael Rhodes, Laurent R. Bellon, Julie Williams, Dawn Madden, Dennis A. Gilbert, Yu N. Wang, Eugene G. Spier, Xiaoqing You, Lily Xu, Jeremy Heil, Stephen Glanowski, Emily S. Winn-Deen, Ivy McMullen, Leila G. Smith
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Publication number: 20120303472Abstract: Methods and systems for ordering assays which detect SNPs or gene expression are provided. The methods use PCR and RT-PCR procedures. Collections of stock assays are assembled using pre- and post-manufacturing quality control procedures and made available to consumers via the Internet. In addition, custom assays are prepared upon order from the consumer and these assays are also prepared using pre- and post-manufacturing quality control procedures. The assays are then delivered to the consumer.Type: ApplicationFiled: April 27, 2012Publication date: November 29, 2012Applicant: APPLIED BIOSYSTEMS, LLCInventors: Ryan T. KOEHLER, Kenneth J. LIVAK, Junko STEVENS, Francisco M. DE LA VEGA, Michael RHODES, Laurent R. BELLON, Janet S. ZIEGLE, Julie WILLIAMS, Dawn MADDEN, Dennis A. GILBERT, Charles R. SCAFE, Hadar I. Isaac, Yu N. WANG, Eugene G. SPIER, Xiaoqing YOU, Lily XU, Jeremy HEIL, Stephen GLANOWSKI, John SCOTT, Emily S. WINN-DEEN, Ivy MCMULLEN, Lini WU, Harold Carey GIRE, Michael W. HUNKAPILLER, Susan EDDINS, Leila G. SMITH
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Publication number: 20110033845Abstract: The teachings relate to methods and kits for detecting whether target nucleic acid sequences are present and/or quantitating target nucleic acid sequences.Type: ApplicationFiled: January 9, 2009Publication date: February 10, 2011Applicant: LIFE TECHNOLOGIES CORPORATION, a Delaware CorporationInventor: Eugene G. SPIER
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Publication number: 20100209932Abstract: The present teachings provide methods for reverse transcribing, and detecting, a plurality of small nucleic acids such as micro RNAs, from the same reaction mixture as a plurality of messenger RNAs. High levels of multiplexing are provided by the use of a plurality of zip-coded stem-loop reverse transcription primers, along with an oligo-dT-promoter-containing reverse transcription primer, in the same reverse transcription reaction mixture. The resulting products can be amplified in an in vitro transcription reaction, and detected on a solid support such as an array. The present teachings also provide compositions, kits, and devices for performing and detecting the reverse transcription reactions described herein.Type: ApplicationFiled: February 16, 2010Publication date: August 19, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventor: EUGENE G. SPIER
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Patent number: 7588896Abstract: The present teachings generally relate to methods, kits, and compositions for detecting target polynucleotide sequences. The teachings also relate to ligation and amplification reactions that generate self-complementary polynucleotide products. In some embodiments, ligation reactions are performed with probes that result in the formation a self-complementary ligation product. Ligation of a hairpin linker to the self-complementary ligation product can form a loop ligation product. In some embodiments, the loop ligation product can be amplified with rolling circle amplification. Detection of a loop ligation product can serve to determine the identity of a target polynucleotide.Type: GrantFiled: January 12, 2007Date of Patent: September 15, 2009Assignee: Applied Biosystems, LLCInventor: Eugene G. Spier
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Patent number: 7485425Abstract: Methods and kits for detecting whether target nucleic acid sequences are present and/or quantitating target nucleic acid sequences are provided.Type: GrantFiled: October 2, 2006Date of Patent: February 3, 2009Assignee: Applied Biosystems Inc.Inventor: Eugene G. Spier
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Publication number: 20080248469Abstract: In some embodiments, the present teachings provide a method of identifying a nucleotide of interest in a target polynucleotide. In some embodiments, the method can comprise forming an amplification strand, wherein the amplification strand comprises a hairpinning end region; hybridizing the hairpinning end region of the amplification strand with a hairpinning region of a target polynucleotide, to form a self-complementary amplification product. After performing an extension reaction, wherein the hairpinning end region of the amplification strand is extended, an extended reaction product is formed. Detection of the extended reaction product can result in the identification of a nucleotide of interest in the target polynucleotide. Additional methods, as well as kits, are also provided.Type: ApplicationFiled: August 14, 2007Publication date: October 9, 2008Applicant: APPLERA CORPORATIONInventor: Eugene G. Spier
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Publication number: 20070196849Abstract: The present teachings provide methods, compositions, and kits for querying the identity of a target polynucleotide strand comprising.Type: ApplicationFiled: February 22, 2007Publication date: August 23, 2007Applicant: Applera CorporationInventor: Eugene G. Spier
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Patent number: 7169561Abstract: The present teachings generally relate to methods, kits, and compositions for detecting target polynucleotide sequences. The teachings also relate to ligation and amplification reactions that generate self-complementary polynucleotide products. In some embodiments, ligation reactions are performed with probes that result in the formation a self-complementary ligation product. Ligation of a hairpin linker to the self-complementary ligation product can form a loop ligation product. In some embodiments, the loop ligation product can be amplified with rolling circle amplification. Detection of a loop ligation product can serve to determine the identity of a target polynucleotide.Type: GrantFiled: December 16, 2005Date of Patent: January 30, 2007Assignee: Applera CorporationInventor: Eugene G. Spier
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Patent number: 7153658Abstract: The present invention relates to methods and kits for detecting the presence or absence of (or quantitating) target nucleic acid sequences using ligation and amplification. The invention also relates to methods, reagents, and kits that employ addressable portions and labeled probes.Type: GrantFiled: September 19, 2003Date of Patent: December 26, 2006Assignee: Applera CorporationInventors: Mark R. Andersen, Michael W. Hunkapiller, Kenneth J. Livak, Eugene G. Spier, H. Michael Wenz
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Patent number: 7085652Abstract: Methods for determining the binding affinity of a probe to a target or targets in a polynucleotide composite using an automated system. Such methods determine relative binding sites based on thermodynamic principles, using a thermodynamic alphabet and a thermodynamic scoring matrix, with appropriate computer software, such as BLASTP. The invention also provides methods for designing polynucleotide probes to be used in hybridization assays, and minimizing the occurrence of cross-hybridization.Type: GrantFiled: February 14, 2003Date of Patent: August 1, 2006Assignee: Applera CorporationInventors: Charles R. Scaf, Nicolas Peyret, Francisco M. De La Vega, Ryan T. Koehler, Eugene G. Spier
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Publication number: 20040121371Abstract: The present invention relates to methods and kits for detecting the presence or absence of (or quantitating) target nucleic acid sequences using ligation and amplification. The invention also relates to methods, reagents, and kits that employ addressable portions and labeled probes.Type: ApplicationFiled: September 19, 2003Publication date: June 24, 2004Applicant: Applera CorporationInventors: Mark R. Andersen, Michael W. Hunkapiller, Kenneth J. Livak, Eugene G. Spier, H. Michael Wenz
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Publication number: 20040018506Abstract: Methods and systems for ordering assays which detect SNPs or gene expression are provided. The methods use PCR and RT-PCR procedures. Collections of stock assays are assembled using pre- and post-manufacturing quality control procedures and made available to consumers via the Internet. In addition, custom assays are prepared upon order from the consumer and these assays are also prepared using pre- and post-manufacturing quality control procedures. The assays are then delivered to the consumer.Type: ApplicationFiled: January 2, 2003Publication date: January 29, 2004Inventors: Ryan T. Koehler, Kenneth J. Livak, Junko Stevens, Francisco M. De La Vega, Michael Rhodes, Laurent R. Bellon, Janet S. Ziegle, Julie Williams, Dawn Madden, Dennis A. Gilbert, Charles R. Scafe, Hadar I. Avi-Itzhak, Yu N. Wang, Eugene G. Spier, Xiaoqing You, Lily Xu, Jeremy Heil, Stephen Glanowski, John Scott, Emily Winn-Deen, Ivy McMullen, Lini Wu, Harold Carey Gire, Susan Eddins, Michael W. Hunkapiller, Leila G. Smith
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Publication number: 20030228591Abstract: Methods for determining the binding affinity of a probe to a target or targets in a polynucleotide composite using an automated system. Such methods determine relative binding sites based on thermodynamic principles, using a thermodynamic alphabet and a thermodynamic scoring matrix, with appropriate computer software, such as BLASTP. The invention also provides methods for designing polynucleotide probes to be used in hybridization assays, and minimizing the occurrence of cross-hybridization.Type: ApplicationFiled: February 14, 2003Publication date: December 11, 2003Inventors: Charles R. Scafe, Nicolas Peyret, Francisco M. De La Vega, Ryan T. Koehler, Eugene G. Spier
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Publication number: 20030190652Abstract: Libraries of assays and methods of compiling the libraries are provided. The assays can identify Single Nucleotide Polymorphisms (SNPs). Methods of validating SNPs are provided. Methods of constructing linkage disequilibrium maps using sets or subsets of SNPs are also provided.Type: ApplicationFiled: January 2, 2003Publication date: October 9, 2003Inventors: Francisco M. De La Vega, Janet S. Ziegle, Hadar I. Avi-Itzhak, Charles R. Scafe, Eugene G. Spier, Yu N. Wang