Patents by Inventor Eugene Zurlo

Eugene Zurlo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20250066415
    Abstract: Methods of producing multiple protein products from blood-based materials including alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins are described herein. The inventive methods include steps of: salt fractionation, chromatography, ultrafiltration, diafiltration, solvent-detergent treatment, and sterile filtration. Advantageously, the inventive methods are simple and produce alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins in high yields. The sequence of process steps can be selected to obtain multiple products from various in-process materials, such as supernatants, pastes, chromatography flow-though, and chromatography washes.
    Type: Application
    Filed: November 12, 2024
    Publication date: February 27, 2025
    Inventors: Eugene Zurlo, Dennis Curtin, David Peter Nowotnik, Charles Heldebrant
  • Publication number: 20250019393
    Abstract: Systems and methods are described in which proteins are isolated from complex solutions in high yield and at high purity. Such systems and methods are carried out at ambient temperature and can be carried out at industrial scale with minimal energy requirements and minimal carbon footprint, using successive chromatographic separations that retain the protein or proteins of interest in flow-through fractions. At least one of the chromatography media used is selected to be capable of interacting with both contaminants and the protein of interest, however capacity of this media is selected such that the protein of interest is displaced and remains in the flow-through. Methods for isolation of IgG, albumin, and both IgG and albumin are provided.
    Type: Application
    Filed: January 30, 2024
    Publication date: January 16, 2025
    Inventors: Eugene Zurlo, Dennis Curtin, Klaus Peter Radtke, Ryan Dorfman, Matthew Whelihan
  • Patent number: 12180250
    Abstract: Methods of producing multiple protein products from blood-based materials including alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins are described herein. The inventive methods include steps of: salt fractionation, chromatography, ultrafiltration, diafiltration, solvent-detergent treatment, and sterile filtration. Advantageously, the inventive methods are simple and produce alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins in high yields. The sequence of process steps can be selected to obtain multiple products from various in-process materials, such as supernatants, pastes, chromatography flow-though, and chromatography washes.
    Type: Grant
    Filed: May 13, 2021
    Date of Patent: December 31, 2024
    Inventors: Eugene Zurlo, David Peter Nowotnik, Charles Heldebrant, Dennis Curtin
  • Patent number: 12037360
    Abstract: Systems and methods are described in which proteins are isolated from complex solution using successive chromatographic separations that retain the protein of interest in the flow-through. At least one of the chromatography media used is selected to be capable of interacting with both contaminants and the protein of interest, however capacity of this media is selected such that the protein of interest is displaced and remains in the flow-through.
    Type: Grant
    Filed: December 22, 2021
    Date of Patent: July 16, 2024
    Inventors: Eugene Zurlo, Dennis Curtin, Klaus Peter Radtke, Ryan Dorfman, Matthew Whelihan
  • Publication number: 20240166685
    Abstract: Systems and methods are described in which proteins are isolated from complex solutions in high yield and at high purity. Such systems and methods are carried out at ambient temperature and can be carried out at industrial scale with minimal energy requirements and minimal carbon footprint, using successive chromatographic separations that retain the protein or proteins of interest in flow-through fractions. At least one of the chromatography media used is selected to be capable of interacting with both contaminants and the protein of interest, however capacity of this media is selected such that the protein of interest is displaced and remains in the flow-through. Methods for isolation of IgG, albumin, and both IgG and albumin are provided.
    Type: Application
    Filed: January 30, 2024
    Publication date: May 23, 2024
    Inventors: Eugene Zurlo, Dennis Curtin, Klaus Peter Radtke, Ryan Dorfman, Matthew Whelihan
  • Publication number: 20240092828
    Abstract: Systems are provided for isolation of a protein, such as immunoglobulin G (IgG), from plasma, where the protein is initially fractioned by salt precipitation, followed by successive ion exchange steps in which the protein appears in unbound, flow-through fractions of the ion exchange steps. Some embodiments employ successive anion exchange steps. Other embodiments employ an anion exchange step followed by application of flow-through of the anion exchange step to a cation exchange step, with the protein collected in flow-through fractions from the cation exchange step. IgG is collected at high yield (typically about 75% or greater) and high purity. Avoidance of binding and elution from chromatography media simplifies processing and scale up without sacrificing IgG quality or yield.
    Type: Application
    Filed: November 21, 2023
    Publication date: March 21, 2024
    Inventors: Eugene Zurlo, Dennis Curtin, Klaus Peter Radtke, Ryan Dorfman, Matthew Whelihan
  • Patent number: 11884702
    Abstract: Methods are provided for isolation of immunoglobulin G (IgG) from plasma, where IgG is initially fractioned by salt precipitation, followed by successive ion exchange steps in which IgG appears in unbound, flow-through fractions of the ion exchange steps. Some embodiments employ successive anion exchange steps. Other embodiments employ an anion exchange step followed by application of flow-through of the anion exchange step to a cation exchange step, with IgG collected in flow-through fractions from the cation exchange step. IgG is collected at high yield (typically about 75% or greater) and high purity. Avoidance of binding and elution from chromatography media simplifies processing and scale up without sacrificing IgG quality or yield.
    Type: Grant
    Filed: December 22, 2021
    Date of Patent: January 30, 2024
    Assignee: Plasma Technologies, LLC
    Inventors: Eugene Zurlo, Dennis Curtin, Klaus Peter Radtke, Ryan Dorfman, Matthew Whelihan
  • Patent number: 11760778
    Abstract: Methods for isolating proteins from solution by precipitation and compositions generated thereby are provided. A nonvolatile precipitation agent is added to an aqueous protein solution at a low concentration. Water is then removed from the resulting solution until the precipitant and the protein content of the solution increase to a concentration that provides the desired segregation of proteins between supernatant and precipitate. Additional water can be removed from the supernatant to provide additional fractionation. Water can be removed by evaporation (e.g. under reduced pressure) and/or diafiltration.
    Type: Grant
    Filed: September 23, 2020
    Date of Patent: September 19, 2023
    Assignee: Plasma Technologies, LLC
    Inventors: Eugene Zurlo, Dennis Curtin, Peter Radtke, Kurt L. Brillhart
  • Patent number: 11739117
    Abstract: Compositions of the inventive concept provide a therapeutic protein with less than 2% contamination by the therapeutic protein in denatured form. Such compositions provide enhanced specific activity and improved stability on storage and/or in serum than corresponding therapeutic protein preparations resulting from conventional isolation methods.
    Type: Grant
    Filed: October 10, 2020
    Date of Patent: August 29, 2023
    Assignee: Plasma Technologies, LLC
    Inventors: Eugene Zurlo, Dennis Curtin, Peter Radtke, Kurt L. Brillhart
  • Patent number: 11732004
    Abstract: Compositions and methods are provided that simplify isolation of proteins of interest from serum or plasma. Finely divided silica or a similar lipid/lipoprotein binding solid is used in combination with a protein precipitating agent to generate a solution that includes the protein of interest and that can be applied to chromatography media without resulting in significant fouling of the media. The method is particularly suitable for isolation of immunoglobulin G.
    Type: Grant
    Filed: April 8, 2021
    Date of Patent: August 22, 2023
    Assignee: Plasma Technologies, LLC
    Inventors: Eugene Zurlo, Dennis Curtin, Klaus Peter Radtke, Kurt L. Brillhart
  • Patent number: 11719690
    Abstract: A method of producing protein products including alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins from plasma includes steps of: (1) adding a salt to the blood product to produce a first intermediate, wherein the salt comprises between 11-13 wt % of the first intermediate; (2) separating the first intermediate to produce a first supernatant and a first paste; (3) adding a salt to the first intermediate to produce a second intermediate, wherein the salt comprises between 21-23 wt % of the second intermediate; (4) separating the second intermediate to produce a second supernatant and a second paste; (5) separating a third intermediate from the second supernatant by affinity chromatography; and (6) separating the third intermediate by ion exchange chromatography to produce an eluate containing the protein product. Advantageously, the inventive methods are simple and produce alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins in high yields.
    Type: Grant
    Filed: September 23, 2020
    Date of Patent: August 8, 2023
    Assignee: Plasma Technologies, LLC
    Inventors: Eugene Zurlo, Dennis Curtin
  • Publication number: 20230129803
    Abstract: Systems and methods are described in which proteins are isolated from complex solution using successive chromatographic separations that retain the protein of interest in the flow-through. At least one of the chromatography media used is selected to be capable of interacting with both contaminants and the protein of interest, however capacity of this media is selected such that the protein of interest is displaced and remains in the flow-through.
    Type: Application
    Filed: December 22, 2021
    Publication date: April 27, 2023
    Inventors: Eugene Zurlo, Dennis Curtin, Klaus Peter Radtke, Ryan Dorfman, Matthew Whelihan
  • Publication number: 20220402969
    Abstract: Methods of producing multiple protein products from blood-based materials including alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins are described herein. The inventive methods include steps of fractionation that utilize a combination of salt and organic solvent. Advantageously, the inventive methods are simple and produce alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins in high yields. The sequence of process steps can be selected to obtain multiple products from various in-process materials, such as supernatants, pastes, chromatography flow-though, and chromatography washes.
    Type: Application
    Filed: August 24, 2022
    Publication date: December 22, 2022
    Inventors: Eugene Zurlo, Dennis Curtin, Klaus Peter Radtke
  • Publication number: 20220204556
    Abstract: Methods are provided for isolation of immunoglobulin G (IgG) from plasma, where IgG is initially fractioned by salt precipitation, followed by successive ion exchange steps in which IgG appears in unbound, flow-through fractions of the ion exchange steps. Some embodiments employ successive anion exchange steps. Other embodiments employ an anion exchange step followed by application of flow-through of the anion exchange step to a cation exchange step, with IgG collected in flow-through fractions from the cation exchange step. IgG is collected at high yield (typically about 75% or greater) and high purity. Avoidance of binding and elution from chromatography media simplifies processing and scale up without sacrificing IgG quality or yield.
    Type: Application
    Filed: December 22, 2021
    Publication date: June 30, 2022
    Inventors: Eugene Zurlo, Dennis Curtin, Klaus Peter Radtke, Ryan Dorfman, Matthew Whelihan
  • Publication number: 20210317163
    Abstract: Compositions and methods are provided that simplify isolation of proteins of interest from serum or plasma. Finely divided silica or a similar lipid/lipoprotein binding solid is used in combination with a protein precipitating agent to generate a solution that includes the protein of interest and that can be applied to chromatography media without resulting in significant fouling of the media. The method is particularly suitable for isolation of immunoglobulin G.
    Type: Application
    Filed: April 8, 2021
    Publication date: October 14, 2021
    Inventors: Eugene Zurlo, Dennis Curtin, Klaus Petter Radtke, Kurt L. Brillhart
  • Publication number: 20210261608
    Abstract: Methods of producing multiple protein products from blood-based materials including alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins are described herein. The inventive methods include steps of: salt fractionation, chromatography, ultrafiltration, diafiltration, solvent-detergent treatment, and sterile filtration. Advantageously, the inventive methods are simple and produce alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins in high yields. The sequence of process steps can be selected to obtain multiple products from various in-process materials, such as supernatants, pastes, chromatography flow-though, and chromatography washes.
    Type: Application
    Filed: May 13, 2021
    Publication date: August 26, 2021
    Inventors: Eugene Zurlo, David Peter Nowotnik, Charles Heldebrant, Dennis Curtin
  • Patent number: 11028125
    Abstract: Methods of producing multiple protein products from blood-based materials including alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins are described herein. The inventive methods include steps of: salt fractionation, chromatography, ultrafiltration, diafiltration, solvent-detergent treatment, and sterile filtration. Advantageously, the inventive methods are simple and produce alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins in high yields. The sequence of process steps can be selected to obtain multiple products from various in-process materials, such as supernatants, pastes, chromatography flow-though, and chromatography washes.
    Type: Grant
    Filed: August 10, 2017
    Date of Patent: June 8, 2021
    Assignee: Plasma Technologies, LLC
    Inventors: Eugene Zurlo, David Peter Nowotnik, Charles Heldebrant, Dennis Curtin
  • Publication number: 20210087228
    Abstract: Methods for isolating proteins from solution by precipitation and compositions generated thereby are provided. A nonvolatile precipitation agent is added to an aqueous protein solution at a low concentration. Water is then removed from the resulting solution until the precipitant and the protein content of the solution increase to a concentration that provides the desired segregation of proteins between supernatant and precipitate. Additional water can be removed from the supernatant to provide additional fractionation. Water can be removed by evaporation (e.g. under reduced pressure) and/or diafiltration.
    Type: Application
    Filed: September 23, 2020
    Publication date: March 25, 2021
    Inventors: Eugene ZURLO, Dennis CURTIN, Peter RADTKE, Kurt L. BRILLHART
  • Publication number: 20210087224
    Abstract: A method for producing a modified cryo-poor precipitate that can be utilized in chromatography without intervening precipitation steps is provided. While thawing frozen plasma at low temperature a precipitating compound (e.g. a salt of an organic acid) is added in small amounts. The resulting modified cryo-poor plasma has a reduced tendency to foul chromatography media, permitting direct application to such media without the need for additional precipitation steps. The resulting modified cryoprecipitate has a higher content of cold-insoluble proteins (such as clotting factors), and can be resolubilized and processed further.
    Type: Application
    Filed: January 9, 2020
    Publication date: March 25, 2021
    Inventors: Eugene ZURLO, Dennis CURTIN, Peter RADTKE, Kurt L. BRILLHART
  • Publication number: 20210087225
    Abstract: Compositions of the inventive concept provide a therapeutic protein with less than 2% contamination by the therapeutic protein in denatured form. Such compositions provide enhanced specific activity and improved stability on storage and/or in serum than corresponding therapeutic protein preparations resulting from conventional isolation methods.
    Type: Application
    Filed: October 10, 2020
    Publication date: March 25, 2021
    Inventors: Eugene ZURLO, Dennis CURTIN, Peter RADTKE, Kurt L. BRILLHART