Patents by Inventor Eui Kyoung JANG

Eui Kyoung JANG has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for preparing DNA oligomer into which single nucleotide is incorporated using terminal deoxynucelotidyl transferase
    Patent number: 11584952
    Abstract: The present invention relates to a method for preparing a DNA oligomer into which a single nucleotide is incorporated using a terminal deoxynucleotidyl transferase. According to the present invention, by using a base hydrolysis reaction or a ribose-borate complex formation method, single incorporation of normal and modified nucleotides in a TdT enzyme-based DNA oligomer modification method can be facilitated. In addition, the method simultaneously provides the usability of TdT and the quantitativeness of modification group incorporation, thereby being effectively usable in the development of a quantitative detection technique or in a crosslinking reaction, both of which require quantitativeness and, according to the present invention, a DNA oligomer, into which a single nucleotide which is a product of a TdT reaction is incorporated, has an exposed a 3? hydroxyl terminus, which is an enzyme recognition site, such that an additional enzymatic technique such as primer extension or ligation can be introduced.
    Type: Grant
    Filed: October 12, 2018
    Date of Patent: February 21, 2023
    Assignee: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION, SEJONG CAMPUS
    Inventors: Seung Pil Pack, Eui Kyoung Jang
  • METHOD FOR PREPARING DNA OLIGOMER INTO WHICH SINGLE NUCLEOTIDE IS INCORPORATED USING TERMINAL DEOXYNUCELOTIDYL TRANSFERASE
    Publication number: 20200340037
    Abstract: The present invention relates to a method for preparing a DNA oligomer into which a single nucleotide is incorporated using a terminal deoxynucleotidyl transferase. According to the present invention, by using a base hydrolysis reaction or a ribose-borate complex formation method, single incorporation of normal and modified nucleotides in a TdT enzyme-based DNA oligomer modification method can be facilitated. In addition, the method simultaneously provides the usability of TdT and the quantitativeness of modification group incorporation, thereby being effectively usable in the development of a quantitative detection technique or in a crosslinking reaction, both of which require quantitativeness and, according to the present invention, a DNA oligomer, into which a single nucleotide which is a product of a TdT reaction is incorporated, has an exposed a 3? hydroxyl terminus, which is an enzyme recognition site, such that an additional enzymatic technique such as primer extension or ligation can be introduced.
    Type: Application
    Filed: October 12, 2018
    Publication date: October 29, 2020
    Applicant: Korea University Research and Business Foundation, Sejong Campus
    Inventors: Seung Pil PACK, Eui Kyoung JANG