Abstract: Provided are a method of screening an inhibitor of caspase activity by lipopolysaccharide and a method of screening a therapeutic agent for inflammatory diseases or sepsis using the same. Accordingly, it is possible to develop a caspase-4-specific inhibitor.

Abstract: Provided are a method of screening an inhibitor of caspase activity by lipopolysaccharide and a method of screening a therapeutic agent for inflammatory diseases or sepsis using the same. Accordingly, it is possible to develop a caspase-4-specific inhibitor.

Abstract: Provided are: a composition for inducing direct conversion from somatic cells into common myeloid progenitor cells, the composition including a chemical cocktail; a method of direct conversion of somatic cells into common myeloid progenitor cells and macrophages by using the composition; common myeloid progenitor cells or macrophages prepared by the method; a pharmaceutical composition for preventing or treating fibrosis or scars, cell therapeutics, a composition for screening drugs, and a 3D printable biomaterial composition for fabricating artificial tissues, each using the common myeloid progenitor cells or the macrophages.

Abstract: Provided are a composition and a kit for reprogramming cancer associated fibroblasts (CAFs) into macrophages, and a method of using the same. According to a method of reprogramming CAFs according to an aspect, macrophages may be prepared with a high yield in a short period of time, and the tumor microenvironment may be suppressed and macrophages produced by reprogramming CAF may elimininate cancer cells. Therefore, the macrophages may be usefully applied as an anticancer agent or an anticancer adjuvant.

Abstract: Provided is a method of producing monomeric caspase activation and recruitment domains (CARDs) in bacteria. According to this method, CARD domains may be easily produced in a monomeric form. Monomeric CARDs may be applied to studies on the mechanism of action of caspase, the development of new drugs, detection and elimination of endotoxins, development of cosmetics, etc.

Abstract: A bacterium that constitutively produces monophosphoryl lipid A (MLA) and a method of producing MLA by using the bacterium may simply produce MLA and a derivative thereof without acid hydrolysis, reduce a probability of natural mutation, and increase yields of MLA and a derivative thereof by constitutive expression of the MLA and derivative thereof.

Abstract: Provided is a method of producing monomeric caspase activation and recruitment domains (CARDs) in bacteria. According to this method, CARD domains may be easily produced in a monomeric form. Monomeric CARDs may be applied to studies on the mechanism of action of caspase, the development of new drugs, detection and elimination of endotoxins, development of cosmetics, etc.

Abstract: A bacterium producing monophosphoryl lipid A (MLA) comprising a genetic modification that increases expression of a gene encoding LpxE polypeptide and a method of producing MLA are provided. According to the present invention, MLA may be produced in a simple manner without acid hydrolysis and/or base hydrolysis.

Abstract: A bacterium that constitutively produces monophosphoryl lipid A (MLA) and a method of producing MLA by using the bacterium may simply produce MLA and a derivative thereof without acid hydrolysis, reduce a probability of natural mutation, and increase yields of MLA and a derivative thereof by constitutive expression of the MLA and derivative thereof.

Abstract: A bacterium producing monophosphoryl lipid A (MLA) comprising a genetic modification that increases expression of a gene encoding LpxE polypeptide and a method of producing MLA are provided. According to the present invention, MLA may be produced in a simple manner without acid hydrolysis and/or base hydrolysis.

Abstract: Provided are a method of culturing an induced pluripotent stem cell and a method of forming a spheroid of the induced pluripotent stem cell. According to an embodiment of the present inventive concept, the induced pluripotent stem cell may have improved stemness.

Abstract: Provided are a composition or a kit for diagnosing colorectal cancer including a CXCL7-measuring agent, and a method of diagnosing colorectal cancer using the same. According to the composition or the kit, and the method, colorectal cancer may be simply diagnosed with high diagnostic accuracy and specificity for colorectal cancer.

Abstract: Provided are technologies capable of direct measurement of activity of a bioactive substance in cell using nanowires, more particularly, a method for measuring intracellular activity of a bioactive substance using a nanowire support to which cells are immobilized and a nanowire support to which target substances for the subject bioactive substance are immobilized, and a chip for measuring intracellular activity of a bioactive substance including nanowires to which cells are immobilized and nanowires to which a target substance for the subject bioactive substance is immobilized.

Abstract: Provided are technologies capable of direct measurement of activity of a bioactive substance in cell using nanowires, more particularly, a method for measuring intracellular activity of a bioactive substance using a nanowire support to which cells are immobilized and a nanowire support to which target substances for the subject bioactive substance are immobilized, and a chip for measuring intracellular activity of a bioactive substance including nanowires to which cells are immobilized and nanowires to which a target substance for the subject bioactive substance is immobilized.

Abstract: An antigen detection kit and an antigen detection method using the same are provided. The antigen detection kit comprises a capture antibody, a detection antibody bound to a single stranded DNA oligonucleotide, a single stranded RNA oligonucleotide complementary sequence to the DNA oligonucleotide, and an RNase.

Abstract: A method for quantitative analysis of interactions between fluorescein-labeled HIF-1? (alpha) C-terminal peptides and cAMP-responsive element binding protein (CBP) or p300 proteins, and a method of screening inhibitors against the formation of HIF-1?-p300 or HIF-1?-CBP protein complexes using the above method is described.

Abstract: The present invention relates to a method for analyzing the interaction between HIF-1 peptide and VBC protein using fluorescence polarization, more precisely, a method for quantitative analysis of formation of HIF-1-VBC protein complex which is composed of the steps of 1) preparing a fluorescent probe by attaching a fluorescein to hydroxyproline containing HIF-1 peptide; 2) reacting the fluorescent probe with VBC protein; and 3) measuring the fluorescence polarization of the above reactant and then comparing the fluorescence polarization with that of the fluorescent probe itself to investigate the changes of fluorescence polarization; a method for screening an inhibitor of the binding of HIF-1 peptide and VBC protein using the above method; and a method for analyzing the activity of prolyl hydroxylase using the above method.

Abstract: The present invention is to prove a new molecular and cellular effect of baicalein, which is selected by a prolyl hydroxylase 2 (PHD2) inhibitor screening method using a compound library. Specifically, the present invention quantitatively analyzed the inhibitory effect of the baicalein against PHD2, confirmed the inhibitory effect against FIH (factor inhibiting HIF), analyzed the HIF (hypoxia inducible factor) protein expression induced by baicalein in a cell, and then, confirmed whether the VEGF is expressed by using reporter assay and ELISA. The above effect of baicalein proves that it can be used for a drug treating ischemic diseases, and, therefore, it can be used for other related diseases.

Abstract: A method and a kit for detecting a target protein in a sample with a signal amplification strategy are provided. The signal amplification strategy is established for the aptamer-based molecular recognition of a target protein with concomitant release of single-stranded DNA (G-DNA), which binds complementarily to a single-stranded RNA comprising a fluorophore and a quencher (“F-RNA-Q”). The fluorescence-quenched RNA is then degraded by RNase H to result in a fluorescence signal, and the undamaged G-DNA is recycled to yield fluorescence amplification.

Abstract: The present invention relates to a biosensor specifically detecting a protein kinase A (PKA), and a diagnosis kit comprising the biosensor. In particular, the present invention relates to a biosensor for detecting a PKA comprising a cantilever, and a protein kinase inhibitor (PKI) immobilized on the surface of the cantilever; and a kit for diagnosing diseases caused by the increase of the activity of a protein kinase, comprising a sample, the biosensor, and a quantification manner.