Abstract: A process for the isolation and separation of lysozyme and avidin from egg white is carried out by:step (a) contacting egg white with a weakly acidic cation exchange resin whereby lysozyme and avidin are adsorbed on to the resin; separating the resin from the egg white and washing the resin to remove residual egg white therefrom; and contacting the washed resin with a low ionic strength eluting buffer whereby lysozyme is eluted from the resin while avidin remains adsorbed on the resin; andstep (b) repeating the complete procedure defined in step (a) for two or more times; andstep (c) finally contacting the resin containing accumulated adsorbed avidin with a high ionic strength eluting buffer whereby avidin is eluted from the resin.Lysozyme and avidin are both commercial products useful, for example, in pharmaceutical applications.