Abstract: A magnetophoresis biochip is provided. The magnetophoresis biochip includes a magnetic force providing unit including a 1-1 surface having magnetic force of a 1-1 pole, and a 2-1 surface which is spaced apart from the 1-1 surface and faces each other and has magnetic force of a 2-1 pole that is opposite to the 1-1 pole, a magnetic force shield including a first magnetic force shield blocking magnetic force of a 1-2 pole of opposite side of surface facing the 2-1 surface in the 1-1 surface, and a second magnetic force shield blocking magnetic force of a 2-2 pole of opposite side of surface facing the 1-1 surface in the 2-1 surface, and a biochip including 3 or more injection channels, a mixing channel and 3 or more separation channels, which are located between the 1-1 surface and the 2-1 surface and extended in one direction and arranged in sequence.