Abstract: A chromatographic method and device for separating one or several organic substances in a liquid sample. According to the method said sample of substances is passed through a chromatographic device containing as separation medium at least one continuous macroporous matrix. The macroporous matrix comprises a cross-linked organic polymer prepared by polymerization of a high internal phase emulsion system of a water-in-oil emulsion of organic monomers, said emulsion containing at least 75% by weight of water phase. The polymerization results in an open porous structure, to allow a convective flow to pass through the macroporous matrix, whereby said organic substances separate from each other and/or the liquid. The pores of the macroporous matrix are unmodified, or surface modified in a manner that the convective flow is not hampered.

Abstract: A liquid chromatography column including a chromatographic matrix, liquid flow inlet means and liquid flow outlet means, and a distributor located adjacent the inlet and functioning to distribute the incoming liquid. The column includes a matrix is that is monolithic and porous; and in that when eluant passes through the matrix a liquid accommodating gap which is devoid of matrix material is present between the matrix and the distributor.