Abstract: The disclosure is directed to binding agents, e.g., a single chain variable fragment (scFv) that specifically binds to a flavivirus NS1 protein, as well as compositions comprising the binding agent and a method of using such compositions to induce an immune response against a flavivirus (e.g., a dengue virus). The disclosure also provides a conjugate comprising the binding agent, and a recombinant NS1 antigen.

Abstract: The present disclosure is directed to antibodies binding to and neutralizing dengue virus and methods for use thereof.

Abstract: The present invention generally relates to cyclodextrin derivatives, including Dexolve®, sulfobutylether beta cyclodextrin interfering with the activity of the viral nonstructural protein NS1 produced by a flavivirus. More particularly, the present invention relates to method of treatment using cyclodextrin compounds for inhibiting or treating a condition resulting from a flavivirus infection, particularly vascular leakage condition and increased virus dissemination.

Abstract: Methods of determining serotype specificity of an antibody that binds to a serotype of a pathogen are provided. Aspects of the methods include producing an assay mixture and evaluating the assay mixture for the presence of an antibody-pathogen-detectable label binding complex to determine the serotype specificity of the antibody. The assay mixture includes the antibody, at least first and second serotypes of the pathogen, a first detectable label that specifically binds to the first serotype of the pathogen, and a second detectable label that specifically binds to the second serotype of the pathogen. The methods find use in a variety of applications, such as for example determining the serotype specificity and/or cross reactivity of an antibody (e.g., an anti-dengue virus antibody).

Abstract: A method and/or system for detecting substances of interest. In specific embodiments, the invention involves a method and/or system using magnetic beads and easily manufactured electrical circuits to detect chemicals and/or substances of interest. In other embodiments, the invention involves a method and/or system for providing a variety of biologic assays. In further embodiments, the invention includes methods and/or systems for an associated device, referred to herein as a dual split-drain transistor.