Abstract: The invention relates to a microscope having an illumination beam path with a radiation source for emitting excitation light, having a detection beam path with a microscope objective and with a detector for detecting emission light emitted by a sample in the sample region as a consequence of being irradiated by the excitation light and having a control unit for evaluating the emission light detected by the detector. The control unit is configured to make use of the original excitation spectra and emission spectra of those dyes with which the sample is prepared, and spectral properties of the illumination beam path and of the detection beam path to ascertain values proportional to concentrations of the respective dyes at a location in the sample from measurement data measured by the detector for the relevant location in the sample for different system configurations. The invention also relates to a microscopy method.

Abstract: The disclosure generally pertains to techniques of providing user assistance when using a microscope to investigate a sample. According to the disclosed techniques, a trained large language model is used to generate the user assistance information. The disclosed techniques enable provisioning of user assistance information that is tailored to the graphical user interface that is used by a user to interact with the microscope.

Abstract: A switch which reduces the voltage between the photocathode and the first dynode in the activated switching state compared to the deactivated switching state and a control unit which is adapted to move a target spot, which can be illuminated by means of the light source, over a scanning field by means of a deflecting unit. The control unit activates the switch when the target spot enters a given region of the scanning field and deactivates the switch when the target spot exits the region.

Abstract: A switch which reduces the voltage between the photocathode and the first dynode in the activated switching state compared to the deactivated switching state and a control unit which is adapted to move a target spot, which can be illuminated by means of the light source, over a scanning field by means of a deflecting unit. The control unit activates the switch when the target spot enters a given region of the scanning field and deactivates the switch when the target spot exits the region.

Abstract: A method for the detection and evaluation of the light generated in a fluorescing specimen by a short pulse laser, wherein at least a first and a second fluorophore and/or a self-fluorescing specimen are separately irradiated with different wavelengths and the specimen light is recorded in a wavelength-dependent manner with at least one nondescanned detector as reference spectrum and a separation into individual spectra is carried out during the irradiation of at least two fluorophores and/or self-fluorescing specimens simultaneously from the measured spectrum and the reference spectra through regression analysis, wherein the wavelength of the short pulse laser is advantageously changed continuously in at least one wavelength region.

Abstract: A method for the detection and evaluation of the light generated in a fluorescing specimen by a short pulse laser, wherein at least a first and a second fluorophore and/or a self-fluorescing specimen are separately irradiated with different wavelengths and the specimen light is recorded in a wavelength-dependent manner with at least one nondescanned detector as reference spectrum and a separation into individual spectra is carried out during the irradiation of at least two fluorophores and/or self-fluorescing specimens simultaneously from the measured spectrum and the reference spectra through regression analysis, wherein the wavelength of the short pulse laser is advantageously changed continuously in at least one wavelength region.