Abstract: The present invention provides nanocarriers for delivering polynucleotide sequences to cells, specifically immune cells, including dendritic cells and methods of use. The methods provide improved delivery and reduced toxicity over prior methods. The method of the present disclosure provide a system for delivering nucleic acids to a cell, consisting of a synthetic PEG-b-PPS-linker-DP polymer for producing nanostructures comprising a poly(ethylene glycol)-blockpoly (propylene sulfide) copolymer (PEG-b-PPS) conjugated with a dendritic-specific branched cationic peptide (DP). The system provides a non-toxic in-vitro method of delivering a polynucleotide to immune cells, including dendritic cells, comprising of contacting the cell in cell culture medium with a nanocarrier wherein the method is non-toxic to the cells.

Abstract: The present disclosure provides methods of adsorbing proteins to the surface of a nanoparticle. Particularly, methods for adsorbing anti-Siglec-6 and anti-Fc?RI antibodies to PPSU nanoparticles, are described. The disclosure further provides compositions comprising the nanoparticles are methods of their use to prevent mast cell secretion and anaphylaxis.

Abstract: Provided herein are rehydratable powdered formulations of nanocarriers that can be used to encompass hydrophobic or hydrophilic cargo. The formulations can be used for medicinal, agricultural, and research applications. Methods of making the formulations are also provided.

Abstract: Provided herein are multi-nanoparticle formulations. Also provided herein are methods for enhancing circulation time and/or cell-targeting efficacy of an effector nanoparticle by administering an endocytosis inhibitory nanoparticle and an effector nanoparticle.