Abstract: Various embodiments of the present disclosure disclose methods and systems for heat exchange in an opto-fluidic instrument. The opto-fluidic instrument includes a sample interface module (SIM), an illumination module, a camera module, and a reagent deck. A coolant from a coolant reservoir is directed into one or more of the modules so that heat exchange occurs at each module with the coolant. The heated coolant from the one or more modules flows to a radiator for cooling, for example, with fans flowing ambient air over the radiator, before the cooled coolant is returned to the coolant reservoir for subsequent use in cooling the modules.

Abstract: An imaging system includes an optical mounting plate having an aperture disposed between a first portion and a second portion, an imaging sensor disposed on the first portion, an objective lens disposed on the second portion, and an illumination assembly arranged within the aperture and disposed between the imaging sensor and the objective lens. The imaging system includes a heat sink coupled to the illumination assembly and configured to transfer heat away from light sources of the illumination assembly via a working fluid that flows through the heat sink.

Abstract: Various embodiments of the present disclosure disclose mitigating internal and external vibrational disturbances in opto-fluidic instrument. In various embodiments, the resonance frequency ranges and/or the vibrational frequency ranges of various modules and components of the opto-fluidic instrument may be selected to avoid resonances from occurring in the opto-fluidic instrument, thereby mitigating vibrations from internal and external sources that may distort the imaging of samples in the opto-fluidic instrument.

Abstract: Systems that include blister packs and methods of use thereof are disclosed. In various embodiments, a system includes a blister pack having at least one blister comprising a base and a top layer. The blister contains a liquid reagent. The system further includes an actuator configured to release the liquid reagent from the blister. The system further includes a substrate configured to hold a tissue sample and receive the liquid reagent from the blister upon release.