Abstract: Disclosed are methods of detecting miRNA expression by an embryo. The methods may be utilized for assessing embryo viability and chromosomal makeup and selecting the embryo for subsequent implantation into a patient. The methods also may be utilized for modifying and optimizing culture media to improve embryo viability via adding one or more miRNAs to culture media for the embryo or depleting one or more miRNAs from the culture media for the embryo. Kits for performing the disclosed methods are also disclosed.

Abstract: Devices and methods are provided for measuring pressure in a non-fluid-filled area of a patient's body. The device includes a capsule having an outer wall and a base surface that define an inner cavity, which retains fluid. The outer wall of the capsule can be made of a flexible material. The device also has a pressure sensor that measures external pressure placed on the outer wall of the capsule. Methods of use include providing the device, positioning the device in the non-fluid-filled area, and measuring pressure within the non-fluid-filled area. The pressure measurement is compared with a predetermined pressure threshold, and a treatment regimen is generated based on the differences between the measured pressure and the predetermined pressure threshold.