Abstract: The present invention relates to methods for the modulation of biofilm formation and antibiotic resistance. Specifically, the present invention identifies the differential expression of biofilm-associated genes in biofilms, relative to their expression in non-biofilm producing bacterial cells. The present invention also identifies the differential expression of biofilm-associated genes in biofilms treated with antibiotic, relative to their expression in untreated biofilms. The present invention describes methods for the diagnostic evaluation of biofilm formation. The invention also provides methods for identifying a compound capable of modulating biofilm formation and antibiotic resistance. The present invention also provides methods for the identification and therapeutic use of compounds as treatments of biofilm-associated diseases or disorders.

Abstract: Novel bacterial quinolone signal molecules and, more particularly, pseudomonas quinolone signal (“PQS”) molecules, e.g., 2-heptyl-3-hydroxy-4-quinolone, and analogs and derivatives thereof are described. Therapeutic compositions containing the molecules, and therapeutic methods, methods of for regulating gene expression, methods for identifying modulators of the autoinducer molecules, and methods of modulating quorum sensing signalling in bacteria using the compounds of the invention are also described.