Abstract: Said vaccine comprises an attenuated strain EPM 10-76 of the virus of canine distemper, obtained from the wild virus, isolated from a mink with distemper, by multiple passage in cell cultures of various origin: dog kidney cell culture, continuous cell culture of the kidneys of human embryo Rh, and a mixed cell culture consisting of dog kidney cells and Japanese quail embryo cells, adapted to the Japanese quail embryo cell culture and grown on this culture.The method of preparing said vaccine consists in inoculating the Japanese quail embryo cell culture with said strain and incubating it on said culture.The advantage of the proposed vaccine is that it is harmless, highly immunogenic and antigenic, as well as it is cheap. The proposed vaccine does not contain extraneous viruses and can be used for both prophylaxis of canine distemper and for eradication of the epizootia. The vaccine in aerosol form can also be used for vaccination.