Patents by Inventor F. C. Thomas Allnutt

F. C. Thomas Allnutt has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240415939
    Abstract: The present disclosure concerns methods and compositions for inhibiting replication of viruses within an organism in need thereof. In some cases, the methods and compositions can be applicable to aquacultured organisms. The aquacultured organisms can include, but are not limited to, crustacean organisms such as shrimp.
    Type: Application
    Filed: June 30, 2021
    Publication date: December 19, 2024
    Inventors: Arun Dhar, F. C. Thomas Allnutt, Roberto Cruz Flores
  • Patent number: 8221767
    Abstract: Infectious pancreatic necrosis virus (IPNV), the etiologic agent of infectious pancreatic necrosis in salmonid fish, causes significant losses to the aquaculture industry. The gene for the viral capsid protein (VP2) was cloned into a yeast expression vector and expressed in Saccharomyces cerevisae. Expression of the capsid gene in yeast resulted in formation of approximately 20 nanometer sub-viral particles composed solely of VP2 protein. Anti-IPNV antibodies were detected in rainbow trout vaccinated either by injection of purified VP2-subviral particles (rVP2-SVP) or by feeding recombinant yeast expressing rVP2-SVP. Challenge of rVP2-SVP immunized trout with a heterologous IPNV strain and subsequent viral load determination showed that both injection and orally vaccinated fish had lower IPNV loads than naive or sham-vaccinated fish.
    Type: Grant
    Filed: December 18, 2007
    Date of Patent: July 17, 2012
    Assignee: Advanced Bionutrition Corporation
    Inventors: Arun K. Dhar, Robert M. Bowers, F. C. Thomas Allnutt
  • Patent number: 8008061
    Abstract: Most microalgae are obligate photoautotrophs and their growth is strictly dependent on the generation of photosynthetically-derived energy. In this study it is shown that the microalga Phaeodaclylurn tricornutum can be engineered to import glucose and grow in the dark through the introduction of genes encoding glucose transporters. Both the human and Chlorella kessleri glucose transporters facilitated the uptake of glucose by P. tricornutum, allowing the cells to metabolize exogenous organic carbon and thrive, independent of light. This is the first successful trophic conversion of an obligate photoautotroph through metabolic engineering, and it demonstrates that methods of cell nourishment can be fundamentally altered with the introduction of a single gene. Since strains transformed with the glucose transport genes are able to grow non-photosynthetically, they can be exploited for the analysis of photosynthetic processes through mutant generation and characterization.
    Type: Grant
    Filed: August 21, 2007
    Date of Patent: August 30, 2011
    Assignee: Martek Biosciences Corporation
    Inventors: Kirk Emil Apt, F. C. Thomas Allnutt, David J. Kyle, James Casy Lippmeier
  • Patent number: 7973148
    Abstract: Methods and constructs for genetic manipulation of one or more of shrimp, shellfish, mollusks, and fish are disclosed. The nucleic acid construct includes a promoter and an internal ribosome entry site of an insect picomavirus, such as a cricket paralysis-like picomavirus. One or more open reading frames can be operably associated with one or both of the promoter and the internal ribosome entry site, and one or more proteins or protein subunits can be expressed upon introduction of the construct into a host cell, such as into a shrimp. Method for producing immortalized crustacean cell lines using enhancer elements derived from shrimp and/or shrimp viruses are also described.
    Type: Grant
    Filed: April 15, 2005
    Date of Patent: July 5, 2011
    Assignee: Advanced Bionutrition Corporation
    Inventors: Arun K. Dhar, F. C. Thomas Allnutt
  • Patent number: 7939710
    Abstract: Most microalgae are obligate photoautotrophs and their growth is strictly dependent on the generation of photosynthetically-derived energy. In this study it is shown that the microalga Phaeodaclylurn tricornutum can be engineered to import glucose and grow in the dark through the introduction of genes encoding glucose transporters. Both the human and Chlorella kessleri glucose transporters facilitated the uptake of glucose by P. tricornutum, allowing the cells to metabolize exogenous organic carbon and thrive, independent of light. This is the first successful trophic conversion of an obligate photoautotroph through metabolic engineering, and it demonstrates that methods of cell nourishment can be fundamentally altered with the introduction of a single gene. Since strains transformed with the glucose transport genes are able to grow non-photosynthetically, they can be exploited for the analysis of photosynthetic processes through mutant generation and characterization.
    Type: Grant
    Filed: April 23, 2001
    Date of Patent: May 10, 2011
    Assignee: Martek Biosciences Corporation
    Inventors: Kirk Emil Apt, F. C. Thomas Allnutt, David J. Kyle, James Casey Lippmeier
  • Patent number: 7932056
    Abstract: A crustacean or rotifer is infected with a recombinant infectious virus that expresses a protein exogenous to the virus. The genome of the crustacean or rotifer itself remains unaltered. Crustacean, rotifer, insect, or viral promoters drive the transcription of a gene inserted into the recombinant virus genome, and the virus replicates in the crustacean or rotifer cell cytoplasm. The infected crustacean or rotifer can be provided directly to humans or non-human animals, or, following production and harvest of the crustaceans or rotifers, purified recombinant protein or polypeptide can be provided. Large quantities of biopharmaceuticals can be produced rapidly and inexpensively using this production system.
    Type: Grant
    Filed: June 23, 2009
    Date of Patent: April 26, 2011
    Assignee: Advanced Bionutrition
    Inventors: Ruth Barratt, F. C. Thomas Allnutt, Robert Bullis, David J. Kyle
  • Patent number: 7550647
    Abstract: A crustacean or rotifer is infected with a recombinant infectious virus that expresses a protein exogenous to the virus. The genome of the crustacean or rotifer itself remains unaltered. Crustacean, rotifer, insect, or viral promoters drive the transcription of a gene inserted into the recombinant virus genome, and the virus replicates in the crustacean or rotifer cell cytoplasm. The infected crustacean or rotifer can be provided directly to humans or non-human animals, or, following production and harvest of the crustaceans or rotifers, purified recombinant protein or polypeptide can be provided. Large quantities of biopharmaceuticals can be produced rapidly and inexpensively using this production system.
    Type: Grant
    Filed: February 17, 2004
    Date of Patent: June 23, 2009
    Assignee: Advanced BioNutrition
    Inventors: Ruth Barratt, F. C. Thomas Allnutt, Robert Bullis, David J. Kyle
  • Patent number: 7256050
    Abstract: In a method for quantitating an analyte by measuring time resolved transfer of fluorescence energy to or from a label quantitatively associated with the analyte, the present invention provides an improvement comprising measuring the energy transferred from donor compounds having the ability to absorb light energy and then transfer this energy to cross-linked allophycocyanin in a time-resolved manner, where the cross-linked allophycocyanin used according to this invention has not been exposed to strongly chaotropic agents after cross-linking.
    Type: Grant
    Filed: June 18, 2001
    Date of Patent: August 14, 2007
    Assignee: Martek Biosciences Corp.
    Inventors: John Peter Morseman, Mark Wesley Moss, F. C. Thomas Allnutt
  • Patent number: 6027900
    Abstract: Genetic fusions for use in genetic engineering of eukaryotic algae employ a promoter from a light harvesting protein fused to a protein of interest. The fusions can be introduced and selected using an antibiotic resistance determinant. One gene useful for such selection is the sh ble gene encoding a bleomycin binding protein.
    Type: Grant
    Filed: October 9, 1998
    Date of Patent: February 22, 2000
    Assignees: Carnegie Institution of Washington, Martek Biosciences Corporation
    Inventors: F. C. Thomas Allnutt, David J. Kyle, Arthur R. Grossman, Kirk R. Apt