Abstract: Vascular access devices and methods of use may utilize a single access port which is attached to a vessel wall and allows for controlled insertion of small to large sized instruments and catheters. The access port may be secured via securement mechanisms deployed from within the vessel lumen. Also, one or more cutting blades may be utilized to create and/or define the individual flaps in the access port and/or underlying tissue wall at the time of port deployment and securement. Such an access port allows a user to access and/or re-access the same artery and/or vein of patients utilizing various diameter catheters and instruments while maintaining hemostasis.