Abstract: The present disclosure provides methods and systems for cell age classification. The method for cell age classification may process images of cells to generate enhanced cell images. The enhanced image of the cell may focus on cell age-dependent phenotypes that may be characteristic of the biological age of the cell. To further improve cell age classification, enhanced cell images may be concatenated and provided to a machine learning-based classifier as an image array and as a single data point. The machine learning-based classifier may use the concatenated enhanced cell images to more accurately determine the age group of the cells. Furthermore, the effects of drug candidates on the biological age of the cells can be determined by contacting the cells of a known chronological age with one or more drug candidates and obtaining images of the cells at a time after the cells have been contacted with the drug candidates.