Abstract: Disclosed herein are novel biomarkers for identifying patients, with cancer, likely to benefit from treatment with an anti-CD25 agent. Also disclosed are methods using said biomarkers for making a treatment decision—or monitoring treatment with an anti-CD25 agent as well as methods of treating a patient, with cancer, comprising administering an anti-CD25 agent based on prior use of the present biomarkers.

Abstract: The invention relates to an image analysis method for determining the biomedical state of a tissue sample. The method comprises: receiving a digital image of a tissue sample; identifying the number and location of A-type cells and B-type cells; obtaining an observed relative distribution; obtaining a reference relative distribution of expected distances between reference A-type cells and reference B-type cells; computing a proximity score as a difference of the reference relative distribution and the observed relative distribution; computing a combined score comprising the proximity score and the density of the A-type cells and/or the density of the B-type cells; using the combined score for determining the biomedical state of the tissue sample and/or outputting the combined score.