Abstract: Disclosed herein are methods, media and other compositions relating to high density cell cultures. Also disclosed are exemplary methods for using high density cell cultures and exemplary products that may be obtained from high density cell cultures.

Abstract: Proteins can be purified and concentrated from crude cell lysates using high-throughput, automated process control systems that use software application programs to instruct one or more processors, controllers, and actuators to control and/or monitor the operations of filtration, purification, and concentration modules in such systems. The high-throughput production of concentrated proteins can be enabled by filtering the lysates and using compressed, non-reactive gas to concentrate the purified proteins, rather than using more complex centrifugation techniques.