Patents by Inventor Fabrice Malergue
Fabrice Malergue has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230288416Abstract: The present invention relates to methods for labeling intracellular and extracellular targets of leukocytes, as well as to kits for performing said methods.Type: ApplicationFiled: March 21, 2023Publication date: September 14, 2023Inventors: Fabrice Malergue, Andreas Van Agthoven
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Patent number: 11666917Abstract: This disclosure is directed to methods and devices (300) associated with Point of Care medical testing and diagnostics. More specifically, methods and devices are described which provide a quick and streamlined way to prepare blood samples for analysis using flow cytometers, microscopes, and other analysis platforms. The benefits include a reduction in the time, resources, and expertise needed for preparing those blood samples without compromising the accuracy and efficacy of diagnosing diseases or identifying specific particulates from those blood samples.Type: GrantFiled: August 31, 2018Date of Patent: June 6, 2023Assignee: Beckman Coulter, Inc.Inventors: Fabrice Malergue, Jean-Marc Busnel
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Patent number: 11635433Abstract: The present invention relates to methods for labeling intracellular and extracellular targets of leukocytes, as well as to kits for performing said methods.Type: GrantFiled: June 21, 2016Date of Patent: April 25, 2023Assignee: Beckman Coulter, Inc.Inventors: Fabrice Malergue, Andreas Van Agthoven
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Publication number: 20210069703Abstract: This disclosure is directed to methods and devices (300) associated with Point of Care medical testing and diagnostics. More specifically, methods and devices are described which provide a quick and streamlined way to prepare blood samples for analysis using flow cytometers, microscopes, and other analysis platforms. The benefits include a reduction in the time, resources, and expertise needed for preparing those blood samples without compromising the accuracy and efficacy of diagnosing diseases or identifying specific particulates from those blood samples.Type: ApplicationFiled: August 31, 2018Publication date: March 11, 2021Inventors: Fabrice Malergue, Jean-Marc Busnel
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Patent number: 10739357Abstract: Systems and methods are provided for determining the age of cellular hemoglobin in individual red blood cells in a blood sample by determining the percentage of HbA1c. In embodiments, a method includes measuring side scatter and fluorescence of the individual red blood cells and identifying immature red blood cells and mature red blood cells from the side scatter and fluorescence measurement. In embodiments, data collected includes the exact number of red blood cells, the fraction limits in fluorescence and side scatter units, the mean value of each fraction in fluorescence and side scatter units, the mean FL1 values per fraction in arbitrary units and the mean side scatter values per fraction in arbitrary units. In embodiments, a method also includes deriving a HbA1c content from the measured mean fluorescence of the individual red blood cells and determining the percentage of HbA1c from the HbA1c content and the hemoglobin content of the red blood cells.Type: GrantFiled: September 11, 2015Date of Patent: August 11, 2020Assignee: Beckman Coulter, Inc.Inventors: Andreas Van Agthoven, Fabrice Malergue, Christophe Godefroy, Enrique Rabellino
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Publication number: 20180231573Abstract: Systems and methods are provided for determining the age of cellular hemoglobin in individual red blood cells in a blood sample by determining the percentage of HbA1c. In embodiments, a method includes measuring side scatter and fluorescence of the individual red blood cells and identifying immature red blood cells and mature red blood cells from the side scatter and fluorescence measurement. In embodiments, data collected includes the exact number of red blood cells, the fraction limits in fluorescence and side scatter units, the mean value of each fraction in fluorescence and side scatter units, the mean FL1 values per fraction in arbitrary units and the mean side scatter values per fraction in arbitrary units. In embodiments, a method also includes deriving a HbA1c content from the measured mean fluorescence of the individual red blood cells and determining the percentage of HbA1c from the HbA1c content and the hemoglobin content of the red blood cells.Type: ApplicationFiled: September 11, 2015Publication date: August 16, 2018Inventors: Andreas VAN AGTHOVEN, Fabrice MALERGUE, Christophe GODEFROY, Enrique RABELLINO
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Patent number: 9678073Abstract: The present invention relates to methods for labeling intracellular and extracellular targets of leukocytes, as well as to kits for performing said methods.Type: GrantFiled: December 21, 2012Date of Patent: June 13, 2017Assignee: Beckman Coulter, Inc.Inventors: Fabrice Malergue, Andreas Van Agthoven
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Publication number: 20160299139Abstract: The present invention relates to methods for labeling intracellular and extracellular targets of leukocytes, as well as to kits for performing said methods.Type: ApplicationFiled: June 21, 2016Publication date: October 13, 2016Inventors: Fabrice MALERGUE, Andreas VAN AGTHOVEN
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Publication number: 20150010923Abstract: The present invention relates to methods for labeling intracellular and extracellular targets of leukocytes, as well as to kits for performing said methods.Type: ApplicationFiled: December 21, 2012Publication date: January 8, 2015Inventors: Fabrice Malergue, Andreas Van Agthoven
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Patent number: 8663935Abstract: Novel compositions containing (i) ligand conjugated phycobiliprotein tandem dyes and (ii) unconjugated phycobiliproteins are provided wherein the phycobiliproteins are derived from the same bacterial or eukaryotic algae species. The phycobiliproteins must be the same or different, but must contain some non-crosslinked subunits which exchange. Also provided are methods for preparing these compositions, kits containing these compositions or components of the same, and methods of using these compositions for cellular and non-cellular analysis.Type: GrantFiled: December 10, 2009Date of Patent: March 4, 2014Assignee: Beckman Coulter, Inc.Inventors: Joseph Wolfers, Fabrice Malergue, Ravinder K. Gupta, Lori A. Charie, Francisco Estevez-Labori, Meryl Forman, Emmanuel Gautherot
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Patent number: 7968279Abstract: A reference control for cell by cell analysis on flow cytometric analyzers contains cellular analogs made of permeated blood cells containing therein aggregated intracellular proteins and preserved antigenic sites thereof, having cellular membrane permeable to antibodies and a suspension medium. The reference control is frozen after being prepared and thawed prior to use. The cellular analogs further contain a fluorescence marker therein. Further disclosed are a method of making the reference control and a method using the reference control, as an internal or stand-alone control, for measurements of cellular hemoglobin and cellular hemoglobin variant of a blood sample.Type: GrantFiled: August 13, 2008Date of Patent: June 28, 2011Assignee: Beckman Coulter, Inc.Inventors: Andreas Van Agthoven, Fabrice Malergue
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Publication number: 20100151493Abstract: Novel compositions containing (i) ligand conjugated phycobiliprotein tandem dyes and (ii) unconjugated phycobiliproteins are provided wherein the phycobiliproteins are derived from the same bacterial or eukaryotic algae species. The phycobiliproteins must be the same or different, but must contain some non-crosslinked subunits which exchange. Also provided are methods for preparing these compositions, kits containing these compositions or components of the same, and methods of using these compositions for cellular and non-cellular analysis.Type: ApplicationFiled: December 10, 2009Publication date: June 17, 2010Applicant: Beckman Coulter, Inc.Inventors: Joseph Wolfers, Fabrice Malergue, Ravinder K. Gupta, Lori A. Charie, Francisco Estevez-Labori, Meryl Forman, Emmanuel Gautherot
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Patent number: 7678578Abstract: A cell permeabilization and stabilization reagent and method of use are disclosed. The reagent contains a N-acyl sarcosine or a salt thereof, a pH adjusting agent to adjust pH of the reagent in a range from about 4 to about 6; and an aqueous medium; the reagent having a low ionic strength defined by a conductivity of less than 9.0 mS/cm. The reagent further contains bovine serum albumin and glycerol. The reagent may further include an alkyl sulfate surfactant. Upon incubating the cells with the reagent, the reagent permeates the cellular membrane to allow penetration of an intracellular marker, causes intracellular protein aggregation within the cellular membrane, while preserves a cellular constituent for binding with a cellular marker for subsequent analysis by flow cytometry.Type: GrantFiled: February 7, 2005Date of Patent: March 16, 2010Assignee: Beckman Coulter, Inc.Inventors: Andreas Van Agthoven, Fabrice Malergue, Enrique Rabellino
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Publication number: 20100041011Abstract: A reference control for cell by cell analysis on flow cytometric analyzers contains cellular analogs made of permeated blood cells containing therein aggregated intracellular proteins and preserved antigenic sites thereof, having cellular membrane permeable to antibodies and a suspension medium. The reference control is frozen after being prepared and thawed prior to use. The cellular analogs further contain a fluorescence marker therein. Further disclosed are a method of making the reference control and a method using the reference control, as an internal or stand-alone control, for measurements of cellular hemoglobin and cellular hemoglobin variant of a blood sample.Type: ApplicationFiled: August 13, 2008Publication date: February 18, 2010Applicant: BECKMAN COULTER, INC.Inventors: Andreas Van Agthoven, Fabrice Malergue
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Publication number: 20090220936Abstract: A method of measuring cellular hemoglobin of a blood sample includes mixing a blood sample with a permeation reagent, and incubating the sample mixture to permeate cellular membrane of red blood cells and to cause hemoglobin aggregation within the cells; adding a neutralization reagent to inhibit further reactions of the permeation reagent; performing a cell-by-cell measurement of side scatter signals of the red blood cells in the sample mixture on a flow cytometer; and obtaining cellular hemoglobin (Hgbcell) of each red blood cell using the obtained side scatter signals. The method further includes measuring cellular hemoglobin of reticulocytes (Hgbretic) by differentiating reticulocytes using a simultaneous fluorescence measurement. The method also includes measuring cellular percentage of a hemoglobin variant in mature red blood cells or reticulocytes by adding a fluorescent antibody in the neutralization reagent and detecting fluorescence signals of antibody bound hemoglobin variant.Type: ApplicationFiled: April 14, 2009Publication date: September 3, 2009Applicant: Beckman Coulter, Inc.Inventors: Andreas Van Agthoven, Fabrice Malergue
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Patent number: 7541190Abstract: A method of measuring cellular hemoglobin of a blood sample includes mixing a blood sample with a permeation reagent, and incubating the sample mixture to permeate cellular membrane of red blood cells and to cause hemoglobin aggregation within the cells; adding a neutralization reagent to inhibit further reactions of the permeation reagent; performing a cell-by-cell measurement of side scatter signals of the red blood cells in the sample mixture on a flow cytometer; and obtaining cellular hemoglobin (Hgbcell) of each red blood cell using the obtained side scatter signals. The method further includes measuring cellular hemoglobin of reticulocytes (Hgbretic) by differentiating reticulocytes using a simultaneous fluorescence measurement. The method also includes measuring cellular percentage of a hemoglobin variant in mature red blood cells or reticulocytes by adding a fluorescent antibody in the neutralization reagent and detecting fluorescence signals of antibody bound hemoglobin variant.Type: GrantFiled: August 9, 2006Date of Patent: June 2, 2009Assignee: Beckman Coulter, Inc.Inventors: Andreas Van Agthoven, Fabrice Malergue
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Publication number: 20090035758Abstract: A method of measuring micronucleated erythrocyte populations is disclosed. The method includes contacting a sample containing erythrocyte populations with a fluorescently labeled antibody specific to immature erythrocytes among erythrocyte populations; adding an aqueous permeation reagent to the sample and incubating the formed permeation sample mixture for an incubation time sufficient to allow the permeation reagent to render cellular membrane of the erythrocyte populations permeable to RNase and dye; adding a RNase reagent to degrade RNA and to inhibit further reaction of permeation reagent; adding a fluorescent nucleic acid dye reagent to stain DNA representing micronuclei in the erythrocyte populations; performing light scatter and fluorescence measurements of the final sample mixture on a flow cytometer; differentiating micronucleated erythrocyte populations from other cell types; and reporting the micronucleated erythrocyte populations of the sample.Type: ApplicationFiled: August 2, 2007Publication date: February 5, 2009Applicant: BECKMAN COULTER, INC.Inventors: Joseph Wolfers, Andreas Van Agthoven, Fabrice Malergue, Michel Herbert
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Publication number: 20070020612Abstract: A method of measuring cellular hemoglobin of a blood sample includes mixing a blood sample with a permeation reagent, and incubating the sample mixture to permeate cellular membrane of red blood cells and to cause hemoglobin aggregation within the cells; adding a neutralization reagent to inhibit further reactions of the permeation reagent; performing a cell-by-cell measurement of side scatter signals of the red blood cells in the sample mixture on a flow cytometer; and obtaining cellular hemoglobin (Hgbcell) of each red blood cell using the obtained side scatter signals. The method further includes measuring cellular hemoglobin of reticulocytes (Hgbretic) by differentiating reticulocytes using a simultaneous fluorescence measurement. The method also includes measuring cellular percentage of a hemoglobin variant in mature red blood cells or reticulocytes by adding a fluorescent antibody in the neutralization reagent and detecting fluorescence signals of antibody bound hemoglobin variant.Type: ApplicationFiled: August 9, 2006Publication date: January 25, 2007Applicant: Beckman Coulter, Inc.Inventors: Andreas Van Agthoven, Fabrice Malergue
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Publication number: 20060178294Abstract: A cell permeabilization and stabilization reagent and method of use are disclosed. The reagent contains a N-acyl sarcosine or a salt thereof, a pH adjusting agent to adjust pH of the reagent in a range from about 4 to about 6; and an aqueous medium; the reagent having a low ionic strength defined by a conductivity of less than 9.0 mS/cm. The reagent further contains bovine serum albumin and glycerol. The reagent may further include an alkyl sulfate surfactant. Upon incubating the cells with the reagent, the reagent permeates the cellular membrane to allow penetration of an intracellular marker, causes intracellular protein aggregation within the cellular membrane, while preserves a cellular constituent for binding with a cellular marker for subsequent analysis by flow cytometry.Type: ApplicationFiled: February 7, 2005Publication date: August 10, 2006Applicant: Beckman Coulter, Inc.Inventors: Andreas Van Agthoven, Fabrice Malergue, Enrique Rabellino
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Patent number: RE45617Abstract: A reference control for cell by cell analysis on flow cytometric analyzers contains cellular analogs made of permeated blood cells containing therein aggregated intracellular proteins and preserved antigenic sites thereof, having cellular membrane permeable to antibodies and a suspension medium. The reference control is frozen after being prepared and thawed prior to use. The cellular analogs further contain a fluorescence marker therein. Further disclosed are a method of making the reference control and a method using the reference control, as an internal or stand-alone control, for measurements of cellular hemoglobin and cellular hemoglobin variant of a blood sample.Type: GrantFiled: March 13, 2013Date of Patent: July 21, 2015Assignee: Beckman Coulter, Inc.Inventors: Andreas Van Agthoven, Fabrice Malergue