Abstract: The invention relates to a strain of fungus having a reduced viscosity, wherein the ID 78713 (GEL3) gene has been invalidated. The invention also relates to the different uses of this strain, as well as to the method of genetic modification.

Abstract: A process for producing cellulolytic and/or hemicellulolytic enzymes with a strain of microorganism belonging to the family of filamentous fungi. The process includes growing the fungi in an aqueous culture medium, in the presence of at least one carbon-based growth substrate, in a stirred and aerated bioreactor. It also includes the production of enzymes, starting with the aqueous culture medium in the presence of at least one inductive carbon-based substrate and also inducing the production of hydrophobins. Further, at least a portion of the hydrophobins produced in step (b) are reintroduced into the growth step (a).

Abstract: The invention relates to a method for separating, from a culture medium, an enzymatic cocktail and the fungus Trichoderma Reesei, the culture medium resulting from enzyme production by the fungus, method in which: said culture medium is subjected, in a period of no longer than 30 h from the halting of production, to a separation on a filter press lined with a fabric having a porosity of 3-20 ?m, so as to obtain a filtrate having a corrected optical density OD at 600 nm of less than 2.5; and the liquid phase obtained is subjected to tangenital micro-filtration on a ceramic membrane having a cut-off limit of 0.5 to 1.4 ?m, so that the corrected OD at 600 nm does not exceed 0.1.

Abstract: The present invention concerns a process for producing enzymes by a strain belonging to a filamentous fungus, said process comprising two steps: (a) a first step of growing the fungi, in the presence of at least one carbon-based growth substrate, in a stirred and aerated bioreactor in batch phase, at a pH of not more than 4.6; (b) a second step of producing enzymes, starting from the culture medium obtained in the first step (a), in the presence of at least one inductive carbon-based substrate, at a pH of not more than 4.6.

Abstract: The present invention relates to a method for producing enzymes by a strain belonging to a filamentous fungus, which comprises three steps: (a) a first step of growing biomass, in the presence of at least one carbon-based growth substrate in a stirred and aerated bioreactor (1) in batch phase, (b) a second step of diluting the culture medium obtained in the first step (a), (c) a third step of producing enzymes from the diluted culture medium obtained in the second step (b), in the presence of at least one inductive carbon-based substrate, in fed-batch phase.

Abstract: The present invention relates to a process for producing cellulolytic and/or hemicellulolytic enzymes with a strain of microorganism belonging to the family of filamentous fungi, said process comprising the following steps: (a) a first step of growing the fungi in an aqueous culture medium, in the presence of at least one carbon-based growth substrate, in a stirred and aerated bioreactor, (b) a second step of producing enzymes, starting with the aqueous culture medium obtained in the first step (a), in the presence of at least one inductive carbon-based substrate, also inducing the production of hydrophobins, and, in a step (d), at least a portion of the hydrophobins produced in step (b) are reintroduced into the growth step (a).

Abstract: The invention relates to a process for producing cellulolytic or hemicellulolytic enzymes comprising A growth phase a) of a cellulolytic microorganism in a closed reactor, in the presence of at least one carbonaceous growth substrate in a concentration between 10 and 90 g/L, at a temperature of 25-30° C. and a pH of 4-5.5 An enzyme production phase b) in which at least one inducer carbonaceous substrate is added, at a temperature of 25-27° C. and a pH of 4-5, process in which the said inducer substrate is a pretreated pomace obtained from a pretreatment process of lignocellulosic material that has not undergone enzymatic hydrolysis and that is added in fed-batch or continuous mode, and which has particular characteristics: a hydrolysis yield greater than 80% in a test and an apparent viscosity, measured in the test, of less than 1 Pa·s for a shear rate of 10 s?1.

Abstract: A process for producing oligosaccharides from lignocellulosic biomass, having the following steps: a) pre-treating the biomass in a pre-treatment reactor (1) in order to provide an effluent containing a pre-treated substrate; b) enzymatic hydrolysis of the pre-treated substrate contained in the effluent from step a) in a reactor, in the presence of cellulases such that a hydrolysate containing glucose, cellulases and water is produced; c) removing at least a portion of the hydrolysate from step b) comprising a liquid fraction; d) reducing the water content of said portion of the hydrolysate in step c) such that the liquid fraction of the hydrolysate has a water content of less than 65% by weight with respect to the total weight of the liquid fraction; e) incubating the hydrolysate from step d) at a temperature in the range 40° C. to 70° C. for the time necessary to produce an effluent enriched in oligosaccharides.

Abstract: The invention relates to a method for separating, from a culture medium, an enzymatic cocktail and the fungus Trichoderma reesei, the culture medium resulting from enzyme production by the fungus, method in which: said culture medium is subjected, in a period of no longer than 30 h from the halting of production, to a separation on a filter press lined with a fabric having a porosity of 3-20 ?m, so as to obtain a filtrate having a corrected optical density OD at 600 nm of less than 2.5; and the liquid phase obtained is subjected to tangenital micro-filtration on a ceramic membrane having a cut-off limit of 0.5 to 1.4 ?m, so that the corrected OD at 600 nm does not exceed 0.1.

Abstract: The process for the production of cellulolytic and/or hemicellulolytic enzymes by a cellulolytic and/or hemicellulolytic microorganism according to the present invention comprises at least one phase for growth in the presence of a source of carbon and at least one phase for production in the presence of an inducing substrate, in which said inducing substrate is a mixture comprising 40% to 65% by weight of glucose or cellulosic hydrolysates, 21% to 25% by weight of lactose and 10% to 39% by weight of xylose or a solution of a lignocellulosic hemicellulosic hydrolysate, the sum of these three constituents being equal to 100%.

Abstract: The object of the invention is a method for modifying the morphology of coagulant type aggregated filamentous fungi comprising: a) the encapsulation of the spores of the said filamentous fungi in the dispersed phase of a water-in-oil emulsion, b) the germination of the encapsulated spores in the said dispersed phase of the emulsion, c) the recovery of the non-aggregated germinated spores, the said emulsion being obtained by using a microfluidic device, d) the culturing of the germinated spores in a liquid medium.

Abstract: The invention relates to a process for producing cellulolytic or hemicellulolytic enzymes comprising A growth phase a) of a cellulolytic microorganism in a closed reactor, in the presence of at least one carbonaceous growth substrate in a concentration between 10 and 90 g/L, at a temperature of 25-30° C. and a pH of 4-5.5 An enzyme production phase b) in which at least one inducer carbonaceous substrate is added, at a temperature of 25-27° C. and a pH of 4-5, process in which the said inducer substrate is a pretreated pomace obtained from a pretreatment process of lignocellulosic material that has not undergone enzymatic hydrolysis and that is added in fed-batch or continuous mode, and which has particular characteristics: a hydrolysis yield greater than 80% in a test and an apparent viscosity, measured in the test, of less than 1 Pa·s for a shear rate of 10 s?1.

Abstract: The invention relates to a process for the production of an enzymatic cocktail from a cellulolytic microorganism producing cellulases and/or hemi-cellulases, comprising: a step for the production of enzymes with a medium being obtained containing enzymes and a microorganism must, said must is separated, or not separated, from the liquid containing said enzymes, a step of cooling said must to a temperature below the temperature of the enzyme production step and for a time such that the beta-glucosidase concentration of the liquid originating from the cooling step is greater than that of the liquid originating from the enzyme production step, and/or the solid volume/total volume ratio is less than said ratio for the enzyme production step, and an enzymatic cocktail is obtained at the end of the cooling step.

Abstract: The invention concerns a process for deactivating a cellulolytic microorganism enabling the production of an enzymatic cocktail, said cocktail being used without separating the cellulolytic microorganism during the biochemical conversion of lignocellulosic materials, comprising at least one step for bringing a gaseous stream into contact with a medium containing said microorganism, said gaseous stream comprising more than 25% by weight of CO2 and comprising less than 0.5 molar % of O2.

Abstract: A process for the production of an enzymatic cocktail with a cellulolytic microorganism comprises two phases: a phase a) for growth of said microorganism in a closed reactor in the presence of a carbonaceous growth solution; a phase b) for the production of said enzymatic cocktail carried out with a supply of carbonaceous production solution the concentration of carbonaceous substrate of which is in the range 150 to 400 g/L, said carbonaceous production solution comprising a carbonaceous inducer substrate; characterized in that said carbonaceous inducer substrate is a liquid residue obtained from a step for pre-treatment of lignocellulosic materials, the C5 sugar oligomers of which represent at least 1% by weight of the total sugars present in said liquid residue, and at least 0.3% by weight of the total sugars present in said carbonaceous production solution.

Abstract: The present invention concerns a process for the production of an enzymatic cocktail by submerged culture with a cellulolytic microorganism, comprising two phases: a phase a) for growth of said microorganism in the presence of at least one carbonaceous growth substrate in a closed reactor, said growth phase being carried out with a concentration of carbonaceous growth substrate in the range 10 to 90 g/L; a phase b) for the production of the enzymatic cocktail, in which at least one carbonaceous inducer substrate is supplied, said carbonaceous inducer substrate being at least one solid residue obtained from the step for enzymatic hydrolysis of lignocellulosic materials which have undergone a pre-treatment step, said production phase being carried out with a concentration of carbonaceous production substrate in the range 150 to 400 g/L.

Abstract: The process for the production of cellulolytic and/or hemicellulolytic enzymes by a cellulolytic and/or hemicellulolytic microorganism according to the present invention comprises at least one phase for growth in the presence of a source of carbon and at least one phase for production in the presence of an inducing substrate, in which said inducing substrate is a mixture comprising 40% to 65% by weight of glucose or cellulosic hydrolysates, 21% to 25% by weight of lactose and 10% to 39% by weight of xylose or a solution of a lignocellulosic hemicellulosic hydrolysate, the sum of these three constituents being equal to 100%.

Abstract: The invention relates to a process for the production of an enzymatic cocktail from a cellulolytic microorganism producing cellulases and/or hemi-cellulases, comprising: a step for the production of enzymes with a medium being obtained containing enzymes and a microorganism must, said must is separated, or not separated, from the liquid containing said enzymes, a step of cooling said must to a temperature below the temperature of the enzyme production step and for a time such that the beta-glucosidase concentration of the liquid originating from the cooling step is greater than that of the liquid originating from the enzyme production step, and/or the solid volume/total volume ratio is less than said ratio for the enzyme production step, and an enzymatic cocktail is obtained at the end of the cooling step.

Abstract: A process for the production of cellulases and hemicellulases using a strain of a filamentous fungus in a stirred and aerated bioreactor, in at least two phases, a phase a) for growth of said strain in the presence of at least one carbonaceous growth substrate in a closed reactor, a phase b) for the continuous production of cellulases, in which at least one carbonaceous inducer substrate is supplied at a supply rate which is constant over a period of at least more than 200 h, said carbonaceous substrate being at least one aqueous hemicellulosic hydrolysate solution obtained from an acid pre-treatment of a lignocellulosic substrate, said aqueous hemicellulosic hydrolysate solution not undergoing prior sterilization and not undergoing pH rectification, being operated at a dilution rate in the range 0.001 to 0.02 h?1.

Abstract: A process for producing oligosaccharides from lignocellulosic biomass, having the following steps: a) pre-treating the biomass in a pre-treatment reactor (1) in order to provide an effluent containing a pre-treated substrate; b) enzymatic hydrolysis of the pre-treated substrate contained in the effluent from step a) in a reactor, in the presence of cellulases such that a hydrolysate containing glucose, cellulases and water is produced; c) removing at least a portion of the hydrolysate from step b) comprising a liquid fraction; d) reducing the water content of said portion of the hydrolysate in step c) such that the liquid fraction of the hydrolysate has a water content of less than 65% by weight with respect to the total weight of the liquid fraction; e) incubating the hydrolysate from step d) at a temperature in the range 40° C. to 70° C. for the time necessary to produce an effluent enriched in oligosaccharides.