Abstract: A method for examining and processing a microscopic sample arranged on a slide includes producing reference markings on the slide by a laser beam of a laser microdissection system. A digital image of the sample and the reference markings on the slide is produced by a digital optical imaging device. An image region is defined and first position information data which indicate a position of the image region is generated. The reference markings are identified in the image and second position information data which indicate a position of the reference markings in the image is generated. The reference markings are identified, and third position information data which indicate the position of the reference markings in the laser microdissection system is generated. The first, second and third position information data are correlated and a sample region which corresponds to the image region is processed.

Abstract: A laser microdissection includes a microscope having an incident-light device, a microscope objective, and a laser unit operable to produce a laser beam having a beam path extending through the incident-light device and through the microscope objective and intersecting an object plane of the microscope objective at an adjustable intersection point. The laser microdissection systems further includes an electrophoresis unit located below the object plane and containing an electrophoresis gel including one or more gel pockets, and a positioning device operable to position the electrophoresis gel in parallel with the object plane of the microscope objective and relative to a defined reference position such that dissectates of a sample that can be arranged in the object plane can be collected in the one or more gel pockets. The electrophoresis unit is operable to be attached by a coupling device. The dissectates are obtained via the laser beam.

Abstract: A method for laser microdissection of a laser microdissection region of a prepared specimen includes driving a holder for the specimen into a holding position using a control device. First and second digital images are captured that depict a same portion of the prepared specimen, with the first image depicting the portion under at least one first microscopic examination method and the second image depicting the portion under at least a second microscopic examination method. A live overlay image is generated of the portion of the prepared image in a live mode. The live overlay is presented on a display area with the images overlaid onto one another. A marking is generated and captured on the live overlay image so as to define the laser microdissection region.

Abstract: A method for calibrating a laser deflection device in a reflected light device of a microscope of a laser microdissection system having a digital image capturing unit comprising an image evaluation module includes generating a laser beam; guiding the laser beam through a microscope objective; directing the laser beam to a position defined by actuation signals; placing a calibration object in the object plane of the microscope objective; actuating the laser deflection device using first actuation signals and first calibration values, making at least one calibration mark on the calibration object; capturing an image of the calibration object by the digital image capturing unit; determining actual position values for the at least one calibration mark: and determining second calibration values based on a relationship between the default position values and the actual position values.

Abstract: A laser microdissectate specimen collector for a laser microdissection device includes a collecting chamber configured to receive a dissectate. The collecting chamber has, on a specimen side, an opening open to the environment for receiving the dissectate. The collecting chamber also has a first valve. The first valve, in a closed state thereof, forms a closure of the collecting chamber opposite to the opening for retaining the dissectate. A capillary line is connected downstream of the first valve to the collecting chamber such that the capillary line is configured to transport the dissectate out of the collecting chamber.

Abstract: A laser microdissection includes a microscope having an incident-light device, a microscope objective, and a laser unit operable to produce a laser beam having a beam path extending through the incident-light device and through the microscope objective and intersecting an object plane of the microscope objective at an adjustable intersection point. The laser microdissection systems further includes an electrophoresis unit located below the object plane and containing an electrophoresis gel including one or more gel pockets, and a positioning device operable to position the electrophoresis gel in parallel with the object plane of the microscope objective and relative to a defined reference position such that dissectates of a sample that can be arranged in the object plane can be collected in the one or more gel pockets. The electrophoresis unit is operable to be attached by a coupling device. The dissectates are obtained via the laser beam.

Abstract: A method for laser microdissection includes detecting at least a portion of an object to be dissected in an image-producing manner in a laser microdissection system and generating a first digital object image. A first processing specification is defined based on the first digital object image. In a first processing step, the object is processed using a laser beam of the laser microdissection system in accordance with the first processing specification. At least a portion of the object is detected in an image-producing manner and a second digital object image is generated. A second processing specification is defined during execution of the first processing step based on the second digital object image. In a second processing step, the object is processed using the laser beam of the laser microdissection system in accordance with the second processing specification.

Abstract: A laser microdissection system includes a microscope that includes a reflected light device having a laser deflector which guides a laser beam provided by a laser through a microscope objective of the microscope onto a sample region for receiving a biological sample and which moves a point of impingement of the laser beam in the sample region. The laser microdissection system also includes a rinsing device comprising fluid dispenser configured to provide a suspension fluid in the sample region and a fluid remover arranged on the rinsing device configured to remove a suspension produced using the suspension fluid from the sample region.

Abstract: A method for calibrating a laser deflection device in a reflected light device of a microscope of a laser inictodissection system having a digital image capturing unit comprising an image evaluation module includes generating a laser beam; guiding the laser beam through a microscope objective; directing the laser beam to a position defined by actuation sinals; placing a calibration object in the object plane of the microscope objective; actuating the laser deflection device using first actuation signais and first calibration values, making at least one calibration mark on the calibration object; capturing an image ofthe calibration object by the digital image capturing unit; determining actual position values for the at least one calibration mark; and determining second calibration values based on a relationship between the default position values and the actual position values.

Abstract: A specimen slide (100) having a sample region (101) in which a sample (200) that can be investigated microscopically can be arranged, and that comprises reference points (A-Z, 1-24, a-j, ?-?) arranged at least in the sample region (101), is proposed. The reference points (A-Z, 1-24, a-j, ?-?) are embodied in such a way that on the basis of an identification of at least a stipulated number of reference points (A-Z, 1-24, a-j, ?-?) in an arbitrary sub-region (102) of the sample region (101), the position of the arbitrary sub-region (102), and/or at least one position therein, on the specimen slide (100) can be unequivocally determined A method for determining and/or retrieving a position on a corresponding specimen slide, and a corresponding sample investigation system, are likewise subjects of the invention.

Abstract: A laser microdissectate specimen collector for a laser microdissection device includes a collecting chamber configured to receive a dissectate. The collecting chamber has, on a specimen side, an opening open to the environment for receiving the dissectate. The collecting chamber also has a first valve. The first valve, in a closed state thereof, forms a closure of the collecting chamber opposite to the opening for retaining the dissectate. A capillary line is connected downstream of the first valve to the collecting chamber such that the capillary line is configured to transport the dissectate out of the collecting chamber.

Abstract: A method for laser microdissection of a laser microdissection region of a prepared specimen includes driving a holder for the specimen into a holding position using a control device. First and second digital images are captured that depict a same portion of the prepared specimen, with the first image depicting the portion under at least one first microscopic examination method and the second image depicting the portion under at least a second microscopic examination method. A live overlay image is generated of the portion of the prepared image in a live mode. The live overlay is presented on a display area with the images overlaid onto one another. A marking is generated and captured on the live overlay image so as to define the laser microdissection region.

Abstract: A specimen slide (100) having a sample region (101) in which a sample (200) that can be investigated microscopically can be arranged, and that comprises reference points (A-Z, 1-24, a-j, ?-?) arranged at least in the sample region (101), is proposed. The reference points (A-Z, 1-24, a-j, ?-?) are embodied in such a way that on the basis of an identification of at least a stipulated number of reference points (A-Z, 1-24, a-j, ?-?) in an arbitrary sub-region (102) of the sample region (101), the position of the arbitrary sub-region (102), and/or at least one position therein, on the specimen slide (100) can be unequivocally determined A method for determining and/or retrieving a position on a corresponding specimen slide, and a corresponding sample investigation system, are likewise subjects of the invention.

Abstract: The invention relates to a slide storage assembly (300) having spacer elements (100, 200) and a slide container (301), which is embodied to accommodate a stack of at least two slide layers, each slide layer comprising a slide (400) and at least two of the spacer elements (100, 200).