Abstract: Provided is a Helicobacter pylori ferritin-based novel coronavirus S protein single-region subunit nano-vaccine. According to the present invention, a receptor binding domain (RBD) of a virus is used as an antigen and is connected with a Helicobacter pylori multimeric protein (HP_Ferritin) to form a fusion protein RBD-HP_Ferritin, such that antigen multimerization is realized; and an eukaryotic cell expression system is then utilized for expression, so as to form a 24-mer nano-antigen by means of the self-assembly action of the HP_Ferritin. According to the solution, the defect that RBD monomers are insufficient in immunogenicity can be overcome; the obtained vaccine can remarkably improve the level of neutralizing antibodies of a host to viruses; and the generated antibodies have the capacity to strongly prevent the viruses from invading target cells.

Abstract: The present application is related to a novel coronavirus S protein double-region subunit nano-vaccine based on a bacterial complex. In the present invention, a receptor binding domain (RBD) and a fusion peptide (FP) of a virus are used together as double antigens, and are connected to a bacterial complex (such as PF_Ferritin or Lumazine Synthase (LS)) to form a fusion protein, so as to achieve antigen multimerization; and then expression is performed by using a eukaryotic cell expression system, and a 24-mer nano-antigen or a 60-mer nano-antigen can be formed by means of self-assembly action. The solution can overcome the defect of insufficient immunogenicity of an RBD monomer. The obtained vaccine can significantly increase the level of a neutralizing antibody against a virus in a host, and the resulting antibody has the capability of strongly blocking a virus from invading a target cell.

Abstract: Disclosed in the present invention is a Helicobacter pylori ferritin-based novel coronavirus S protein double-region subunit nanovaccine. According to the present invention, both a receptor binding domain (RBD) and a fusion peptide (FP) of a virus are taken as double antigens and are connected with a Helicobacter pylori multimeric protein (HP_Ferritin) to form a fusion protein RBD-FP-HP_Ferritin, so that antigen multimerization is realized; and an eukaryotic cell expression system is then utilized for expression, so as to form a 24-mer nano-antigen by means of the self-assembly action of the HP_Ferritin. According to the solution, the defect that RBD monomers are insufficient in immunogenicity can be overcome; the obtained vaccine can remarkably improve the level of neutralizing antibodies of a host to viruses; and the generated antibodies have the capacity to strongly prevent the viruses from invading target cells.

Abstract: The present invention relates to a VC-CAR molecule and a use thereof in removing HIV-1 infected cells. The VC-CAR molecule is characterized by linking an HIV-1 broad-spectrum neutralizing antibody VRC01 sourced scFv sequence and an intracellular domain sequence of a conventional CAR molecule which are respectively used as extracellular and intracellular domains. A T cell modified by the VC-CAR molecule can be specifically activated and secrete a great number of cytotoxicity-related cytokines, so that lysis of a target cell can be powerfully mediated, and it can be better used for anti-infection adoptive immunotherapy.

Abstract: The present invention relates to a VC-CAR molecule and a use thereof in removing HIV-1 infected cells. The VC-CAR molecule is characterized by linking an HIV-1 broad-spectrum neutralizing antibody VRC01 sourced scFv sequence and an intracellular domain sequence of a conventional CAR molecule which are respectively used as extracellular and intracellular domains. A T cell modified by the VC-CAR molecule can be specifically activated and secrete a great number of cytotoxicity-related cytokines, so that lysis of a target cell can be powerfully mediated, and it can be better used for anti-infection adoptive immunotherapy.

Abstract: A high-power bi-directional non-recovery spring magnetic valve including permanent magnets has an upper magnetic circuit part and lower magnetic circuit part symmetrically disposed and connected together via an armature connection rod and a housing connection ring, the upper magnetic circuit part comprises an upper iron core, an upper housing, an upper coil, an upper annular permanent magnet, an upper yoke iron and an upper push rod, the lower magnetic circuit part comprises a lower iron core, a lower housing, a lower coil, a lower annular permanent magnet, a lower yoke iron and a lower push rod. The magnetic valve comprising permanent magnets realizes bistable-state magnetic retaining, and has an adjustable retaining force for permanent magnet adjustment, quick response, and high output force.

Abstract: A high-power bi-directional non-recovery spring magnetic valve including permanent magnets has an upper magnetic circuit part and lower magnetic circuit part symmetrically disposed and connected together via an armature connection rod and a housing connection ring, the upper magnetic circuit part comprises an upper iron core, an upper housing, an upper coil, an upper annular permanent magnet, an upper yoke iron and an upper push rod, the lower magnetic circuit part comprises a lower iron core, a lower housing, a lower coil, a lower annular permanent magnet, a lower yoke iron and a lower push rod. The magnetic valve comprising permanent magnets realizes bistable-state magnetic retaining, and has an adjustable retaining force for permanent magnet adjustment, quick response, and high output force.