Abstract: An intermediate of biliverdin, and preparation method and use thereof includes applying a compound represented by formula 2 as a raw material; R is hydrogen, C1-C5 alkyl, or benzyl; “” at positions A and B independently represent a single bond or a double bond; when “” represents a single bond, R1 or R2 connected to the single bond is selected from one of tosyl, p-toluenesulfonyl, phenylsulfonyl, phenylsulfinyl; and when “” represents a double bond, R1 or R2 connected to the double bond is hydrogen.

Abstract: A pyrrolinone compound represented by formula 1. R1 is selected from one of C1-C5 alkoxy, benzyloxy, C1-C5 alkyl, or a phenyl group; R2, R3 at each occurrence independently represent hydrogen, C1-C5 alkyl, C1-C5 alkoxy, C1-C5 alkylthio, C1-C5 alkane sulfinyl, C1-C5 alkane sulfonyl, a substituted phenyl, a substituted phenoxy, a substituted phenylthio, a substituted phenylene sulfinyl, or a substituted benzene sulfonyl; and n1, n2 at each occurrence independently are an integer from 1 to 5.

Abstract: The disclosure provides a dipyrrolidine-1-one compound represented by formula 1; R is hydrogen, C1-C5 alkyl, or benzyl; R1 is selected from thiophenyl, phenylsulfinyl, phenylsulfonyl, p-toluene sulfide, p-tosylsulfinyl, and tosyl; and R2 is selected from methyl, alkoxycarbonyl, formyl, acetoxymethyl, carboxyl, and hydrogen. The compound is prepared by a compound represented by formula 2 and a compound represented by formula 3 through a condensation reaction.

Abstract: A method for preparing biliverdin or a derivative thereof includes applying a compound represented by formula 2 as a raw material; R is hydrogen, C1-C5 alkyl, or benzyl; “” at positions A and B independently represent a single bond or a double bond; when “” represents a single bond, R1 or R2 connected to the single bond is selected from one of tosyl, p-toluenesulfonyl, phenylsulfonyl, phenylsulfinyl; and when “” represents a double bond, R1 or R2 connected to the double bond is hydrogen.

Abstract: A method of preparing bilirubin IX? from biliverdin IX? diester, the method including: 1) adding sodium hydroxide dissolved in a first methanol solution to biliverdin IX? diester dissolved in a second methanol solution, to yield a first mixture; adding water to the first mixture to hydrolyze biliverdin IX? diester; adding an acid to the first mixture to adjust the pH value thereof; removing a first solvent of the first mixture through rotary evaporation, and removing an inorganic salt through rinsing, and vacuum drying, to yield biliverdin IX?; and 2) dissolving the biliverdin IX? in an alcoholic solution, and adding a radical scavenger and borohydride to the alcoholic solution, to yield a second mixture; adding a ketone to the second mixture to decompose excess borohydride; adding the acid to the second mixture to adjust the pH value thereof; and removing a second solvent of the second mixture through rotary evaporation.

Abstract: A method of preparing bilirubin IX? from biliverdin IX? diester, the method including: 1) adding sodium hydroxide dissolved in a first methanol solution to biliverdin IX? diester dissolved in a second methanol solution, to yield a first mixture; adding water to the first mixture to hydrolyze biliverdin IX? diester; adding an acid to the first mixture to adjust the pH value thereof; removing a first solvent of the first mixture through rotary evaporation, and removing an inorganic salt through rinsing, and vacuum drying, to yield biliverdin IX?; and 2) dissolving the biliverdin IX? in an alcoholic solution, and adding a radical scavenger and borohydride to the alcoholic solution, to yield a second mixture; adding a ketone to the second mixture to decompose excess borohydride; adding the acid to the second mixture to adjust the pH value thereof; and removing a second solvent of the second mixture through rotary evaporation.