Patents by Inventor Faqing WU

Faqing WU has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Genetically engineered strain with high yield of L-valine and method for producing L-valine by fermentation
    Patent number: 11802300
    Abstract: A genetically engineered strain having high-yield of L-valine is disclosed. Starting from Escherichia coli W3110, an acetolactate synthase gene alsS of Bacillus subtilis is inserted into a genome thereof and overexpressed; a ppGpp 3?-pyrophosphate hydrolase mutant R290E/K292D gene spoTM of Escherichia coli is inserted into the genome and overexpressed; a lactate dehydrogenase gene ldhA, a pyruvate formate lyase I gene pflB, and genes frdA, frdB, frdC, frdD of four subunits of fumaric acid reductase are deleted from the genome; a leucine dehydrogenase gene bcd of Bacillus subtilis replaces a branched chain amino acid transaminase gene ilvE of Escherichia coli; and an acetohydroxy acid isomeroreductase mutant L67E/R68F/K75E gene ilvCM replaces the native acetohydroxy acid isomeroreductase gene ilvC of Escherichia coli. Furthermore, the L-valine fermentation method is improved by using a two-stage dissolved oxygen control. The L-valine titer and the sugar-acid conversion rate are increased.
    Type: Grant
    Filed: October 30, 2019
    Date of Patent: October 31, 2023
    Assignee: TIANJIN UNIVERSITY OF SCIENCE & TECHNOLOGY
    Inventors: Xixian Xie, Heyun Wu, Jiachu Wang, Faqing Wu, Xiaoqian Liu, Yanan Hao
  • GENETICALLY ENGINEERED STRAIN WITH HIGH YIELD OF L-VALINE AND METHOD FOR PRODUCING L-VALINE BY FERMENTATION
    Publication number: 20230107828
    Abstract: A genetically engineered strain having high-yield of L-valine is disclosed. Starting from Escherichia coli W3110, an acetolactate synthase gene alsS of Bacillus subtilis is inserted into a genome thereof and overexpressed; a ppGpp 3?-pyrophosphate hydrolase mutant R290E/K292D gene spoTM of Escherichia coli is inserted into the genome and overexpressed; a lactate dehydrogenase gene ldhA, a pyruvate formate lyase I gene pflB, and genes frdA, frdB, frdC, frdD of four subunits of fumaric acid reductase are deleted from the genome; a leucine dehydrogenase gene bcd of Bacillus subtilis replaces a branched chain amino acid transaminase gene ilvE of Escherichia coli; and an acetohydroxy acid isomeroreductase mutant L67E/R68F/K75E gene ilvCM replaces the native acetohydroxy acid isomeroreductase gene ilvC of Escherichia coli. Furthermore, the L-valine fermentation method is improved by using a two-stage dissolved oxygen control. The L-valine titer and the sugar-acid conversion rate are increased.
    Type: Application
    Filed: October 30, 2019
    Publication date: April 6, 2023
    Applicant: TIANJIN UNIVERSITY OF SCIENCE & TECHNOLOGY
    Inventors: Xixian XIE, Heyun WU, Jiachu WANG, Faqing WU, Xiaoqian LIU, Yanan HAO