Abstract: Novel microbial host strains are provided which are transformed by a vector molecule comprising a DNA fragment encoding a lipolytic enzyme and a marker for selection, capable of producing active lipase. Said DNA fragment is preferably derived from a Pseudomonas species.

Abstract: Novel microbial host strains are provided which are transformed by a vector molecule comprising a DNA fragment encoding a lipolytic enzyme and a marker for selection, capable of producing active lipase. Said DNA fragment is preferably derived from a Pseudomonas species.

Abstract: The oxidative deterioration of water-containing products for human consumption, e.g. beverages and oil and/or fat based products is minimized by introduction of immobilized yeast. Yeast is immobilized in and/or on a solid material which allows only very slow penetration by water. Thin layers material of such a yeast-bearing solid, e.g. paraffin, wax, can be applied to the lining of crown corks. The yeast will retain sufficient viability, even after pasteurization of the contents of a container closed with the crown cork. The yeast will minimize the oxygen concentration in the contents between the time of pasteurization and eventual consumption of the contents.

Abstract: Novel microbial host strains are provided which are transformed by a vector molecule comprising a DNA fragment encoding a lipolytic enzyme and a marker for selection, capable of producing active lipase. Said DNA fragment is preferably derived from a Pseudomonas species.

Abstract: Novel lipolytic enzymes are provided having an optimum pH in the range of 8 and 10.5 which exhibit effective lipase activity in an aqueous solution containing a detergent at a concentration of up to about 10 g/l under washing conditions at a temperature of 60.degree. C. or below and at a pH between 7 and 11. These lipases are useful in washing compositions, premixes and enzymatic detergent additives. They are obtainable from bacteria strains selected from Pseudomonas pseudoalcaligenes, Pseudomonas stutzeri and Acinetobacter calcoaceticus. Preferred strains are Ps. pseudoalcaligenes CBS 467.85, CBS 468.85, CBS 471.85, CBS 473.85 and ATCC 29625, Ps. stutzeri CBS 461.85 and Acinetobacter calcoaceticus CBS 460.85, and variants and mutants thereof.

Abstract: A food-grade oxygen scavenger for removing oxygen from foods and beverages in containers is prepared by immobilizing dried yeast containing at least 92% dry matter in a solid material such as wax or paraffin. The solid material allows for very slow penetration of water and permits the yeast to contact only water that penetrates therethrough. The immobilized yeast is coated on an inside surface of a container or on a surface of a closure such as a stopper that is on the inside of the container when closed. After adding a food or beverage and closing the container, the immobilized yeast removes oxygen from the container. The closed container and its contents can be pasteurized and the yeast retain sufficient viability to remove oxygen.

Abstract: Novel lipolytic enzymes having an optimum pH in the range of 8 and 10.5 which exhibits effective lipase activity in an aqueous solution containing a detergent at a concentration of up to about 10 g/l under washing conditions at a temperature of 60.degree. C. or below and at a pH between 7 and 11. These lipases are useful in washing compsitions, premixes and enzymatic detergent additives. They are obtainable from bacteria strains selected from Psedomonas pseudoalcaligenes, Pseudomanas stutzeri and Acinetobacter calcoaceticus. Preferred strains are Pxs. pseudoalcaligenes CBS 467.85, CBS 468.85, CBS 471.85, CBS 473.85 and ATCC 29625, Ps. stutzeri CBS 461.85 and Acimetobacter calcoaceticus CBS 460.85, and variants and mutants thereof.