Abstract: The present disclosure provides agronomically stable liquid agricultural compositions, methods of formulation thereof, and methods of application thereof. The agricultural compositions comprise nitrogen-fixing microorganisms and one or more of a buffering agent, a microbial stabilizer, and a physical stabilizer. The disclosed liquid agricultural compositions have a longer shelf life and greater ease of application than other existing dry and liquid formulations. The disclosed liquid agricultural compositions are stable for a period of thirty days or longer with low toxin accumulation and high microbial stability. The compositions are suitable for use on agricultural plant tissues or the environs thereof for providing a source of fixed atmospheric nitrogen to the agricultural plant. The compositions are used to increase crop yield and decrease yield variance.

Abstract: The present disclosure provides the integration of exogenous polymers to microbes to confer increased stability and viability for an extended shelf life of the desired microbes (e.g., bacteria), as compared to those microbes lacking the exogenous polymers. The microbes include transgenic microbes, non-transgenic microbes, and non-intergeneric remodeled microbes. The utilization of the taught microbial products will enable a significant expansion of the typical shelf life of microbial compositions. The microbes comprising exogenous polymers described herein are able to be combined with other agriculturally beneficial compositions. Furthermore, the disclosure provides for the addition of exogenous microbial biofilms to the aforementioned compositions.

Abstract: The present disclosure provides the integration of exogenous microbial biofilms to confer increased stability and viability for an extended shelf life of desired microbes (e.g., bacteria), as compared to those microbes in the absence of the exogenous microbial biofilms. The microbes include transgenic microbes, non-transgenic microbes, and non-intergeneric remodeled microbes. The utilization of the taught microbial products will enable a significant expansion of the typical shelf life of microbial compositions. The microbes comprising exogenous biofilms taught herein are able to be combined with other agriculturally beneficial compositions.

Abstract: Production-scale solid state bioreactors designed to facilitate and maximize the automation of solid-state fermentation processes while maintaining sterile transfer of materials. The invention also provides automated solid-state fermentation systems that include the bioreactors and related methods of solid state fermentation utilizing the bioreactors.