Patents by Inventor Federico Katzen
Federico Katzen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240101999Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).Type: ApplicationFiled: August 17, 2023Publication date: March 28, 2024Inventors: Thomas POEHMERER, Phillip KUHN, Frank NOTKA, Andreas ZEIDLER, Korbinian HEIL, Axel TREFZER, Geir FONNUM, Federico KATZEN, Kristian ANDERSSON, Xiquan LIANG
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Publication number: 20210047636Abstract: The invention generally relates to compositions and methods for designing and producing functional DNA binding effector molecules and associated customized services, tool kits and functional assays. In some aspects, the invention provides methods and tools for efficient assembly of customized TAL effector molecules. Furthermore, the invention relates to uses of TAL effector molecules and functional evaluation of such TAL by, for example, customized assays.Type: ApplicationFiled: June 29, 2020Publication date: February 18, 2021Inventors: Federico KATZEN, Jonathan CHESNUT, Frank NOTKA, Matthias ARNOLD, Kevin HOFF, Stephen WHITESIDE
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Publication number: 20200325514Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).Type: ApplicationFiled: January 7, 2020Publication date: October 15, 2020Inventors: Todd PETERSON, Axel TREFZER, Thomas POEHMERER, Federico KATZEN, Xiquan LIANG
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Patent number: 10563240Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).Type: GrantFiled: March 14, 2014Date of Patent: February 18, 2020Assignees: LIFE TECHNOLOGIES CORPORATION, THERMO FISHER SCIENTIFIC GENEART GMBHInventors: Todd Peterson, Axel Trefzer, Thomas Poehmerer, Federico Katzen, Xiquan Liang
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Publication number: 20200010827Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).Type: ApplicationFiled: July 22, 2019Publication date: January 9, 2020Inventors: Thomas Poehmerer, Phillip Kuhn, Frank Notka, Andreas Zeidler, Korbinian Heil, Axel Trefzer, Geir Fonnum, Federico Katzen, Kristian Andersson, Xiquan Liang
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Patent number: 10407676Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).Type: GrantFiled: December 9, 2015Date of Patent: September 10, 2019Assignees: LIFE TECHNOLOGIES CORPORATION, THERMO FISHER SCIENTIFIC GENEART GMBH, LIFE TECHNOLOGIES ASInventors: Thomas Poehmerer, Phillip Kuhn, Frank Notka, Andreas Zeidler, Korbinian Heil, Axel Trefzer, Geir Fonnum, Federico Katzen, Kristian Andersson
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Publication number: 20190112596Abstract: The invention generally relates to compositions and methods for designing and producing functional DNA binding effector molecules and associated customized services, tool kits and functional assays. In some aspects, the invention provides methods and tools for efficient assembly of customized TAL effector molecules. Furthermore, the invention relates to uses of TAL effector molecules and functional evaluation of such TAL by, for example, customized assays.Type: ApplicationFiled: April 12, 2018Publication date: April 18, 2019Inventors: Federico KATZEN, Jonathan CHESTNUT, Frank NOTKA, Matthias Arnold, Kevin HOFF, Stephen WHITESIDE
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Publication number: 20180245116Abstract: The present disclosure generally relates to compositions and methods for the assembly of nucleic acid molecules into larger nucleic acid molecules. Also provided are compositions and methods for seamlessly connection of nucleic acid molecules with high sequence fidelity.Type: ApplicationFiled: January 17, 2018Publication date: August 30, 2018Inventors: Xiquan LIANG, Chang-Ho BAEK, Lansha PENG, Federico KATZEN
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Publication number: 20160264962Abstract: The invention generally relates to compositions and methods for designing and producing functional DNA binding effector molecules and associated customized services, tool kits and functional assays. In some aspects, the invention provides methods and tools for efficient assembly of customized TAL effector molecules. Furthermore, the invention relates to uses of TAL effector molecules and functional evaluation of such TAL by, for example, customized assays.Type: ApplicationFiled: July 28, 2015Publication date: September 15, 2016Inventors: Federico KATZEN, Jonathan Chesnut, Frank Notka, Matthias Arnold, Kevin Hoff, Stephen Whiteside
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Publication number: 20160186166Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).Type: ApplicationFiled: December 9, 2015Publication date: June 30, 2016Inventors: Thomas POEHMERER, Phillip Kuhn, Frank Notka, Andreas Zeidler, Korbinian Heil, Axel Trefzer, Geir Fonnum, Federico Katzen, Kristian Andersson, Xiquan Liang
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Publication number: 20160122792Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).Type: ApplicationFiled: March 14, 2014Publication date: May 5, 2016Inventors: Todd PETERSON, Axel TREFZER, Thomas POEHMERER, Federico KATZEN, Xiquan LIANG
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Publication number: 20160017394Abstract: The present disclosure generally relates to compositions and methods for the assembly of nucleic acid molecules into larger nucleic acid molecules. Also provided are compositions and methods for seamlessly connection of nucleic acid molecules with high sequence fidelity.Type: ApplicationFiled: July 15, 2015Publication date: January 21, 2016Inventors: Xiquan LIANG, Chang-Ho Baek, Lansha Peng, Federico Katzen
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Publication number: 20130274129Abstract: The invention generally relates to compositions and methods for designing and producing functional DNA binding effector molecules and associated customized services, tool kits and functional assays. In some aspects, the invention provides methods and tools for efficient assembly of customized TAL effector molecules. Furthermore, the invention relates to uses of TAL effector molecules and functional evaluation of such TAL by, for example, customized assays.Type: ApplicationFiled: April 4, 2013Publication date: October 17, 2013Inventors: Federico KATZEN, Jonathan Chesnut, Frank Notka, Matthias Arnold, Kevin Hoff, Stephen Whiteside
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Publication number: 20120190064Abstract: Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of a feeding solution that includes amino acids and an energy source to an ongoing in vitro synthesis system. The invention also includes methods of using a high-yield in vitro synthesis system to produce large quantities of proteins with incorporated labeled amino acids for analysis by methods such as by NMR. The invention further includes vectors for enhanced production of proteins from nucleic acid templates using in vitro synthesis systems.Type: ApplicationFiled: January 23, 2012Publication date: July 26, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Wieslaw Antoni KUDLICKI, Shiranthi Keppetipola, Julia Fletcher, Ashley Elaine Getbehead, Federico Katzen, Laura Vozza-Brown
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Publication number: 20110195450Abstract: In vitro protein synthesis systems and methods are provided that produce membrane proteins in soluble form. In some aspects, the invention provides methods of synthesizing proteins using in vitro protein synthesis systems that include an apolipoprotein, in which higher yields of soluble protein are produced than in the absence of the apolipoprotein. Apolipoproteins useful in the present invention include naturally occurring apolipoproteins, as well as sequence variants of wild-type apolipoproteins, and engineered apolipoproteins. The apolipoproteins can be provided in an in vitro protein synthesis system associated with lipid or not associated with lipid. The invention also provides compositions and kits for synthesis of proteins in soluble form, in which the compositions and kits include cell extracts for protein translation and at least one apolipoprotein biomolecule.Type: ApplicationFiled: July 30, 2010Publication date: August 11, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: WIESLAW KUDLICKI, JULIA FLETCHER, FEDERICO KATZEN
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Publication number: 20110104781Abstract: Systems and methods are provided for producing a protein of interest that is typically not amenable to expression in soluble form in in vitro expression systems. In some aspects, the invention provides methods of synthesizing proteins using in vitro protein synthesis systems that include a scaffold protein such as apolipoprotein or an amphipathic alpha helix containing (“AAHC”) protein, in which higher yields of soluble protein are produced than in the absence of the scaffold protein. The scaffold proteins may be provided in an in vitro protein synthesis system associated with lipid or not associated with lipid. The scaffold protein may be provided as a protein per se or may be encoded by a nucleic acid template and co-expressed with the protein of interest. The invention also provides compositions and kits for synthesis of proteins in soluble form, in which the compositions and kits include cell extracts for protein expression and isolation.Type: ApplicationFiled: September 24, 2010Publication date: May 5, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: FEDERICO KATZEN, JULIA FLETCHER, WIESLAW KUDLICKI, JOSEPH BEECHEM, LILIN WANG
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Publication number: 20100233782Abstract: Systems and methods are provided for producing a protein of interest that is typically not amenable to expression in soluble form in in vitro expression systems. In some aspects, the invention provides methods of synthesizing proteins using in vitro protein synthesis systems that include a scaffold protein such as apolipoprotein or an amphipathic alpha helix containing (“AAHC”) protein, in which higher yields of soluble protein are produced than in the absence of the scaffold protein. The scaffold proteins may be provided in an in vitro protein synthesis system associated with lipid or not associated with lipid. The scaffold protein may be provided as a protein per se or may be encoded by a nucleic acid template and co-expressed with the protein of interest. The invention also provides compositions and kits for synthesis of proteins in soluble form, in which the compositions and kits include cell extracts for protein expression and isolation.Type: ApplicationFiled: October 9, 2009Publication date: September 16, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Federico Katzen, Julia Fletcher, Wieslaw Kudlicki, Joseph Beechem, Lilin Wang
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Publication number: 20090209032Abstract: Compositions, methods and kits for in vitro systems for synthesis of biomolecules such as polypeptides, are provided herein. Cell extracts that provide enhanced yields of soluble proteins using in vitro protein synthesis methods are provided. The invention also includes methods for producing high yields of proteins by the addition of a feeding solution that includes amino acids and an energy source to an ongoing in vitro synthesis system. The invention also includes methods of using a high-yield in vitro synthesis system to produce large quantities of proteins with incorporated labeled amino acids for analysis by methods such as by NMR. The invention further includes vectors for enhanced production of proteins from nucleic acid templates using in vitro synthesis systems.Type: ApplicationFiled: September 15, 2008Publication date: August 20, 2009Applicant: INVITROGEN CORPORATIONInventors: Wieslaw Antoni Kudlicki, Shiranthi Keppetipola, Julia Fletcher, Ashley Elaine Getbehead, Federico Katzen, Laura Vozza-Brown
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Publication number: 20090161828Abstract: Systems and methods are provided for producing a protein of interest that is typically not amenable to expression in soluble form in in vitro expression systems. In some aspects, the invention provides methods of synthesizing proteins using in vitro protein synthesis systems that include a scaffold protein such as apolipoprotein or an amphipathic alpha helix containing (“AAHC”) protein, in which higher yields of soluble protein are produced than in the absence of the scaffold protein. The scaffold proteins may be provided in an in vitro protein synthesis system associated with lipid or not associated with lipid. The scaffold protein may be provided as a protein per se or may be encoded by a nucleic acid template and co-expressed with the protein of interest. The invention also provides compositions and kits for synthesis of proteins in soluble form, in which the compositions and kits include cell extracts for protein expression and isolation.Type: ApplicationFiled: December 11, 2008Publication date: June 25, 2009Applicant: LIFE TECHNOLOGIESInventors: Federico Katzen, Julia Fletcher, Wieslaw Kudlicki, Joseph Beechem, Lilin Wang
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Publication number: 20080248565Abstract: Systems and methods are provided for producing a protein of interest that is typically not amenable to expression in soluble form in in vitro expression systems. In some aspects, the invention provides methods of synthesizing proteins using in vitro protein synthesis systems that include a scaffold protein such as apolipoprotein or an amphipathic alpha helix containing (“AAHC”) protein, in which higher yields of soluble protein are produced than in the absence of the scaffold protein. The scaffold proteins may be provided in an in vitro protein synthesis system associated with lipid or not associated with lipid. The scaffold protein may be provided as a protein per se or may be encoded by a nucleic acid template and co-expressed with the protein of interest. The invention also provides compositions and kits for synthesis of proteins in soluble form, in which the compositions and kits include cell extracts for protein expression and isolation.Type: ApplicationFiled: February 29, 2008Publication date: October 9, 2008Applicant: INVITROGEN CORPORATIONInventors: Federico Katzen, Julia Fletcher, Wieslaw Kudlicki, Joseph Beechem, Lilin Wang