Patents by Inventor Federico Melelli

Federico Melelli has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Process for preserving the phosphorylating activity of yeast, applied to the production of fructose-1,6-diphosphate
    Patent number: 4925801
    Abstract: A process for preserving the phosphorylating activity of brewer's yeast by means of the intracellular immobilization of the glycolytic enzymes by glutaraldehyde. The process provides the following steps:permeabilization of the cell wall by the combined effect of temperature and the osmotic shock caused by the addition of a concentrated solution of dextrose and phosphates;immobilization of the glycolytic enzymes by cross-linking with intracellular proteins;protection of the SH groups against possible oxidation by the addition of deproteinized yeast extract.
    Type: Grant
    Filed: March 23, 1987
    Date of Patent: May 15, 1990
    Inventors: Guillermo Bisso, Federico Melelli
  • Process for the continuous production of fructose-1,6-di-phosphate by employ of immobilized yeast
    Patent number: 4920049
    Abstract: The process provides the enzymatic phosphorylation of glucose to FDP by means of yeast immobilized by glutaraldehyde and the separation of FDP by ultrafiltration. A suspension of glutaraldehyde-treated yeast is fed to a fermenter, with addition of a nutrient mixture consisting of dextrose (1M), sodium phosphate (0.5 M), magnesium chloride (10 mM) and phosphoric acid until pH 6.5. The mixture is re-circulated through a hollow fiber ultrafiltration unit to separate the FDP that forms during the phosphorylation reaction.
    Type: Grant
    Filed: July 15, 1987
    Date of Patent: April 24, 1990
    Inventors: Guglielmo M. Bisso, Federico Melelli