Abstract: Provided are a helicase mutant, a preparation method therefor, and use thereof in high-throughput sequencing. The helicase mutant has at least one cysteine on a surface of a three-dimensional structure of an amino acid sequence as set forth in SEQ ID NO: 1, the at least one cysteine being substituted with alanine. The cysteine is C133, C164, C292, C323, and/or C347. Preferably, the amino acid sequence of the helicase mutant is as set forth in SEQ ID NO: 6. The helicase mutant exhibits excellent DNA unwinding activity, good protein uniformity, reduced cross-linking interactions between protein molecules, enhanced adaptability with perforin, improved sequencing stability, as well as integrity and continuity of electrical signals. Consequently, it achieves a better sequencing performance. It can be utilized for the control and characterization of nucleic acids and applied in single-molecule nanopore sequencing.

Abstract: A chimeric DNA polymerase includes: a first fragment having at least 80% homology to at least part of an N-end domain of KOD DNA polymerase; a second fragment having at least 80% homology to at least part of an exonucleolytic domain of Pab DNA polymerase; a third fragment having at least 80% homology to at least part of the N-end domain of KOD DNA polymerase; a fourth fragment having at least 80% homology to at least part of a palm domain of Pfu DNA polymerase; a fifth fragment having at least 80% homology to at least part of a finger domain of Pab DNA polymerase; a sixth fragment having at least 80% homology to at least part of the palm domain of Pfu DNA polymerase; and a seventh fragment having at least 80% homology to at least part of a thumb domain of KOD DNA polymerase.

Abstract: The present application relates to the field of test devices, and in particular to a test device for sealing material under complex vibration. The test device includes a test body, a vibration component, and a test assembly.

Abstract: A test device for bubble damage of sealing material includes first radius measure members, first location measure members and second location measure members on a first housing along a circumferential direction. The first radius measure members may move along a height direction of the first housing, the first location measure members may move along a radial direction of the first housing, and the second location measure members may move along the height direction of the first housing. The device further includes a plurality of second radius measure members on a second housing along a circumferential direction. The second radius measure members may move along a radial direction of the second housing. A sample to be measured may be placed in the housings, and the device may be used to test for bubbles on a top surface or a side surface of the sample.

Abstract: A performance testing device for the piston rod sealing assembly includes a bracket, a support apparatus, a drive apparatus and a leakage detection apparatus, where the support apparatus includes a cylinder body that is internally provided with a movable channel, and the movable channel is configured to allow a piston rod equipped with a sealing component to pass therethrough; the drive apparatus is provided on the bracket and configured to be connected with the piston rod; the leakage detection apparatus includes a limit member and an oil receiver, the limit member is provided with a detection channel and an oil channel and connected to the cylinder body, the detection channel is communicated with the movable channel and configured to allow the piston rod to pass therethrough; one end of the oil channels is communicated with the detection channel and the other end thereof is communicated with the oil receiver.

Abstract: The disclosed air core inductor array includes a top plate positioned in a first plane and a bottom plate positioned in a second plane. The disclosed air core inductor array additionally includes a plurality of air core inductors arranged between the top plate and the bottom plate, wherein the top plate and the bottom plate form a mutual inductor. Various other methods, systems, and computer-readable media are also disclosed.

Abstract: Methods and systems related to chip-to-chip electrical connection interfaces are disclosed. The electrical connections can be formed between chiplets, chip-scale packages, or unpackaged die. The electrical connection can serve as a physical connection to support various interface protocols such as the Bunch of Wires (BoW) or Universal Chiplet Interconnect Express (UCIe) protocols. An electrical connection interface comprises a first die connection for a first die, a first tuning block, a first escape region connection coupling the first die connection to the first tuning block, a second die connection for a second die, a second tuning block, a second escape region connection coupling the second die connection to the second tuning block, and a main trace coupling the first tuning block to the second tuning block.

Abstract: The disclosed electrical circuit can include a circuit connection structure and a pad provided to the circuit connection structure. The disclosed electrical circuit can additionally include an inductive tuning element provided to the circuit connection structure. The inductive tuning element can compensate parasitic capacitance of the pad. Various other methods, systems, and computer-readable media are also disclosed.

Abstract: The present application relates to the field of test device, and provides a partitioned contact temperature cycle test bench for seal material performance, including a temperature control device, a support device and a drive device, where the temperature control device includes a first thermostatic module and a second thermostatic module, the first thermostatic module is provided with a first thermal conduction surface, the second thermostatic module is provided with a second thermal conduction surface, the first thermal conduction surface and the second thermal conduction surface enclose a receive cavity, and temperature of the first thermostatic module is different from temperature of the second thermostatic module; the support device supports a piece to be tested, the support device is rotatably provided in the receive cavity, the support device is provided with a thermal conduction portion, the thermal conduction portion is in contact with the piece to be tested, and the thermal conduction portion is in c

Abstract: The present disclosure relates to voltage regulator circuits, low dropout regulator chips, chip systems, and electronic devices. An example voltage regulator circuit includes a voltage input end, a voltage output end, a first voltage end, a second voltage end, an LDO, a first comparator, a first delay circuit, and a plurality of first switch circuits. The LDO is configured to receive a reference voltage at the voltage input end, and output a first voltage. The voltage output end is configured to receive the first voltage, and output a second voltage. The first comparator is configured to receive a first threshold voltage at the first voltage end and the second voltage, and output a first comparison result.

Abstract: The present disclosure provides a measuring device and measuring method for a shaft-holding force of a seal, relating to the technical field of sealing. The measuring device of a shaft-holding force of a seal includes a driving assembly, a first pressing plate, a pressure sensor, an adiabatic rod, a conical shaft, a first support, a second support, a test box and a seal, a working temperature of the seal may be simulated by the test box, the conical shaft is located inside the test box, the pressure sensor is located outside the test box, the adiabatic rod is connected between the pressure sensor and the conical shaft, and the pressure sensor and the conical shaft are in different environments. Through separating the pressure sensor from the test box by the adiabatic rod, a temperature of the test box will not affect the pressure sensor.

Abstract: Embodiments of the present disclosure provides a projection lens and a projecting device; wherein, the projection lens comprises a front lens group, a rear lens group and a stop, sequentially from an object side to an image side along the same optical axis, wherein the stop is located between the front lens group and the rear lens group; wherein the front lens group has a focal length of f11 satisfying: 40 mm<f11<60 mm; the rear lens group has a focal length of f22 satisfying: 2 mm<f22<12 mm.

Abstract: Provided are methods for producing 3,6-disubstituted-imidazo[1,2-b]pyridazine compounds or the salts thereof.

Abstract: Provided is a chimeric DNA polymerase, including: a first to seventh peptide segments, which have at least 80% homology with at least part of: the amino acid sequence in the N-terminal domain of a 9°N DNA polymerase; the amino acid sequence in the exonucleolytic domain of a KOD DNA polymerase; the amino acids in the N-terminal domain of the 9°N DNA polymerase; the amino acids in the palm domain of a KOD DNA polymerase; the amino acids in the finger domain of the Pfu DNA polymerase; the amino acids in the palm domain of the KOD DNA polymerase; and the amino acids in the thumb domain of the 9°N DNA polymerase, respectively.

Abstract: The disclosed computer-implemented method for automatically generating a printed circuit board design includes executing, by at least one processor based on input parameters, a grouping operation that identifies network shapes defining regions occupied by corresponding networks for one or more layers of an integrated circuit package. The method additionally includes identifying, by the at least one processor based on the identified network shapes, connections of the integrated circuit package. The method also includes automatically generating, by the at least one processor and based on the identified connections, a printed circuit board design. Various other methods, systems, and computer-readable media are also disclosed.

Abstract: A data management method includes obtaining, by a network device, first network operation data collected at a first frequency and second network operation data collected at a second frequency higher than the first frequency. The data management method also includes sending, by the network device to a control device, the first network operation data. The data management method further includes, in response to an abnormality occurring in the first network operation data or the second network operation data, sending, by the network device, third network operation data to the control device. The third network operation data includes network operation data collected at the first frequency or the second frequency before a moment of the abnormality.

Abstract: A semiconductor device includes a power delivery device die stack including a plurality of vertically arranged power delivery device dies. The plurality of power delivery device dies including at least a first power delivery device die and a second power delivery device die electrically connected to the first power delivery device die. The semiconductor device includes at least one external interconnect for providing a power input to the power delivery device die stack and at least one external interconnect for supplying a power output from the power delivery device die stack.

Abstract: The present invention relates to a nucleic acid molecule capable of blocking motor protein, a library containing the nucleic acid molecule, a method for constructing the nucleic acid molecule or library containing the same, and an application of the nucleic acid molecule or library containing same.

Abstract: Systems, methods, and apparatus are described herein for maintaining high signal integrity and high bandwidth in data transmissions between an integrated circuit package and a PCB. The integrated circuit package has multiple layers. Signal pins for connecting the integrated circuit package, both physically and electrically, to the PCB are located at a first layer. A signal trace is coupled to each signal pin. The signal trace bifurcates into two branches and couples to a given signal pin at the distal end of each branch.

Abstract: Provided is helicase BCH1X, which comprises an amino acid sequence represented by SEQ ID NO: 1 or 2. Further provided are a complex structure comprising helicase BCH1X and a binding moiety used for binding to a polynucleotide, and a use thereof in the control and characterization of a polynucleotide and in single molecule nanopore sequencing.