Abstract: Implementations of the present invention provide a digital twin intelligent edge terminal and an operation method therefor pertaining to the field of digital twin terminals. The terminal comprises: a state perception module physically connected to a cloud digital twin system and single or multiple application objects and sensors via various data interfaces; a decision reception and execution module configured to perform the dispatch of decision information according to whether a control system for controlling application objects has an instruction interface and a transmission protocol; a human-computer interaction module; a digital twin local control module interacting with the cloud digital twin system or collaborating with the state perception module, the decision reception and execution module, and the human-computer interaction module in different working modes; and a functional module manager implementing information interaction between the modules.

Abstract: A digital twin (DT) enhanced method and system for detecting and compensating complex equipment, including: firstly intelligently deciding detection strategies that adapt to personalized scenarios based on a complex equipment and operating scenario fused DT model; then, autonomously implementing the detection strategies based on a complex equipment and detection device fused DT model, and location and quantitative errors according to detection results; and finally, deciding and implementing a compensation solution fused with an operating process based on a complex equipment and controller fused DT model.

Abstract: The present invention discloses a bacterium and an obtaining method and application thereof. The bacterium has a property of coproducing 1,3-propanediol and D-lactic acid. Further, the bacterium is Klebsiella oxytoca, including Klebsiella oxytoca PDL-5 CCTCC M 2016185. The obtaining method of the bacterium may be to obtain the bacterium by directly screening wild bacteria that satisfy conditions from the environment or performing gene engineering modification to wild bacteria. The present invention has the advantages that the bacteria can coproduce 1,3-propanediol and D-lactic acid through fermentation, the molar conversion rate and the concentration of the two products are very high, the types of byproducts are few, the concentration is low, the product extraction process is simplified, the high-efficiency biological production of 1,3-propanediol and D-lactic acid can be realized, and the industrial application prospect is very great.

Abstract: Provided are a genetically engineered strain for producing polylactic acid and a method for producing polylactic acid. The genome of the genetically engineered strain is integrated with a coding sequence of exogenous D-lactate dehydrogenase gene, a coding sequence of exogenous propionyl-CoA transferase gene, and a coding sequence of exogenous polyhydroxyalkanoate synthase gene, enabling the genetically engineered strain to express exogenous D-lactate dehydrogenase, exogenous propionyl-CoA transferase, and exogenous polyhydroxyalkanoate synthase. The method includes: providing the above genetically engineered strain of Synechococcus elongatus; introducing carbon dioxide and culturing the genetically engineered strain under light; and when a growth OD of the genetically engineered strain reaches the maximum, collecting and drying the genetically engineered strain, and recycling the polylactic acid in the strain.

Abstract: A digital twin data model driven high-performance virtual simulation method and system, including: based on a product design process, paying attention to variable operating conditions or variable product structures to obtain three kinds of data relevant to a design product, relevant products, or operating conditions: sensor data, mechanism simulation data, and fusion data obtained by correcting the mechanism simulation data with the sensor data; processing the obtained data, reversely resolving a running mechanism according to a data driven algorithm, and building a data model driven high-performance virtual simulation model; and based on new operating conditions of the design product or improved new products, calling the built data model driven high-performance virtual simulation model to obtain the virtual simulation results. The method and system can be used for performance analysis and prediction on design products instead of modeling simulation and physical experiments.

Abstract: The present invention discloses a bacterium and an obtaining method and application thereof. The bacterium has a property of coproducing 1,3-propanediol and D-lactic acid. Further, the bacterium is Klebsiella oxytoca, including Klebsiella oxytoca PDL-5 CCTCC M 2016185. The obtaining method of the bacterium may be to obtain the bacterium by directly screening wild bacteria that satisfy conditions from the environment or performing gene engineering modification to wild bacteria. The present invention has the advantages that the bacteria can coproduce 1,3-propanediol and D-lactic acid through fermentation, the molar conversion rate and the concentration of the two products are very high, the types of byproducts are few, the concentration is low, the product extraction process is simplified, the high-efficiency biological production of 1,3-propanediol and D-lactic acid can be realized, and the industrial application prospect is very great.

Abstract: A genetically engineered bacterial strain that produces N-acetylglucosamine, as well as a method of construction and use thereof. The genetically engineered bacterial strain can ferment N-acetylglucosamine under a condition of 40-50° C. Through knocking out genes for glucosamine 6-phosphate deaminase, N-acetylglucosamine-6-phosphate deacetylase and the N-acetylglucosamine transporter protein from a parental bacterium, an N-acetylglucosamine catabolism pathway is blocked. Moreover, overexpression genes for glucosamine 6-phosphate synthase and glucosamine 6-phosphate acetylase are introduced, enabling extra cellular accumulation of N-acetylglucosamine and high-temperature fermentation of N-acetylglucosamine at a temperature higher than 40° C.

Abstract: Provides an artificial enzyme obtained by improving upon a sequence of a natural nicotine dehydrogenase, wherein the improvement comprises replacing at least one amino acid hindering product release with an amino acid with smaller side chains, thereby improving a catalytic rate.

Abstract: The present invention discloses a bacterium and an obtaining method and application thereof. The bacterium has a property of coproducing 1,3-propanediol and D-lactic acid. Further, the bacterium is Klebsiella oxytoca, including Klebsiella oxytoca PDL-5 CCTCC M 2016185. The obtaining method of the bacterium may be to obtain the bacterium by directly screening wild bacteria that satisfy conditions from the environment or performing gene engineering modification to wild bacteria. The present invention has the advantages that the bacteria can coproduce 1,3-propanediol and D-lactic acid through fermentation, the molar conversion rate and the concentration of the two products are very high, the types of byproducts are few, the concentration is low, the product extraction process is simplified, the high-efficiency biological production of 1,3-propanediol and D-lactic acid can be realized, and the industrial application prospect is very great.

Abstract: Provides an artificial enzyme obtained by improving upon a sequence of a natural nicotine dehydrogenase, wherein the improvement comprises replacing at least one amino acid hindering product release with an amino acid with smaller side chains, thereby improving a catalytic rate.

Abstract: The present invention discloses a bacterium and an obtaining method and application thereof. The bacterium has a property of coproducing 1,3-propanediol and D-lactic acid. Further, the bacterium is Klebsiella oxytoca, including Klebsiella oxytoca PDL-5 CCTCC M 2016185. The obtaining method of the bacterium may be to obtain the bacterium by directly screening wild bacteria that satisfy conditions from the environment or performing gene engineering modification to wild bacteria. The present invention has the advantages that the bacteria can coproduce 1,3-propanediol and D-lactic acid through fermentation, the molar conversion rate and the concentration of the two products are very high, the types of byproducts are few, the concentration is low, the product extraction process is simplified, the high-efficiency biological production of 1,3-propanediol and D-lactic acid can be realized, and the industrial application prospect is very great.

Abstract: The present invention provides D-lactate dehydrogenase, an engineered strain containing the D-lactate dehydrogenase and a construction method and use of the engineered strain. The present invention discloses a D-lactate dehydrogenase which has unique properties and is from Thermodesulfatator indicus, and the D-lactate dehydrogenase has good thermophily and heat stability. By using the D-lactate dehydrogenase and the gene engineering reconstruction method, a fermentation product of the reconstructed Bacillus licheniformis can be redirected to optically-pure D-lactic acid with a high yield from naturally produced 2,3-butanediol, and the optical purity of the produced D-lactic acid reaches 99.9%; and raw materials for fermentation are low-cost, and a fermentation state is between an anaerobic fermentation state and a microaerobic fermentation state.

Abstract: The present invention provides D-lactate dehydrogenase, an engineered strain containing the D-lactate dehydrogenase and a construction method and use of the engineered strain. The present invention discloses a D-lactate dehydrogenase which has unique properties and is from Thermodesulfatator indicus, and the D-lactate dehydrogenase has good thermophily and heat stability. By using the D-lactate dehydrogenase and said gene engineering reconstruction method, a fermentation product of the reconstructed Bacillus licheniformis can be redirected to optically-pure D-lactic acid with a high yield from naturally produced 2,3-butanediol, and the optical purity of the produced D-lactic acid reaches 99.9%; and raw materials for fermentation are low-cost, and a fermentation state is between an anaerobic fermentation state and a microaerobic fermentation state.

Abstract: The Invention discloses a preparation method for a triptyl polymer separation membrane, wherein, it comprises the following steps: take triptycene compound containing active group, dichloride compound and diamino compound containing ether bond as monomers, and conduct polymerization in aprotic organic solvent in the presence of acid-binding agent; pour the obtained polymer solution into deionized water for precipitation after the reaction, filter out precipitates and wash with methanol and dry to get triptyl polymer; dissolve the triptyl polymer in aprotic organic solvent to produce a membrane-casting solution, apply the solution on support and dry to get a triptyl polymer separation membrane. The Invention solves the problem that the reticular polymer material is insoluble, and breaks the restriction for application of such material in separation membrane due to its insoluble and aging features.

Abstract: Provided herein is D-lactate dehydrogenase, an engineered strain containing the D-lactate dehydrogenase, and a construction method and use of the engineered strain. The D-lactate dehydrogenase has unique properties and is from Thermodesulfatator indicus, and the D-lactate dehydrogenase has good thermophily and heat stability. By using the D-lactate dehydrogenase and the gene engineering reconstruction method, a fermentation product of the reconstructed Bacillus licheniformis can be redirected to optically-pure D-lactic acid with a high yield from naturally produced 2,3-butanediol, and the optical purity of the produced D-lactic acid reaches 99.9%; and raw materials for fermentation are low-cost, and a fermentation state is between an anaerobic fermentation state and a microaerobic fermentation state.

Abstract: The present invention is directed to water-soluble membrane proteins, methods for the preparation thereof and methods of use thereof.

Abstract: The present invention is directed to a computer implemented method for executing a procedure to select a water-soluble variant of a G Protein-Coupled Receptor (GPCR).

Abstract: The present invention discloses a bacterium and an obtaining method and application thereof. The bacterium has a property of coproducing 1,3-propanediol and D-lactic acid. Further, the bacterium is Klebsiella oxytoca, including Klebsiella oxytoca PDL-5 CCTCC M 2016185. The obtaining method of the bacterium may be to obtain the bacterium by directly screening wild bacteria that satisfy conditions from the environment or performing gene engineering modification to wild bacteria. The present invention has the advantages that the bacteria can coproduce 1,3-propanediol and D-lactic acid through fermentation, the molar conversion rate and the concentration of the two products are very high, the types of byproducts are few, the concentration is low, the product extraction process is simplified, the high-efficiency biological production of 1,3-propanediol and D-lactic acid can be realized, and the industrial application prospect is very great.

Abstract: Provides an artificial enzyme obtained by improving upon a sequence of a natural nicotine dehydrogenase, wherein the improvement comprises replacing at least one amino acid hindering product release with an amino acid with smaller side chains, thereby improving a catalytic rate.

Abstract: Systems and methods for analyzing a driver's brake behavior are provided. In some aspects, the system includes a memory that stores instructions for executing processes for analyzing the driver's brake behavior, and a processor configured to execute the instructions. In aspects, the processes include: receiving data from one or more multi-modal sensors, determining whether each braking event of a plurality of braking events is a hard braking event; analyzing the one or more physiological signals of the driver to determine whether each of the hard braking events is a reactive hard braking event or an intended hard braking event; applying a Lasso regression analysis and a classification framework for each of the hard braking events to identify features that indicate whether a respective hard braking event is a reactive hard braking event or an intended hard braking event; and generating a signal notifying a vehicle of the features.