Abstract: A method and a system of molecular typing and subtyping classifier for immune-related diseases are provided. The method includes: conducting molecular typing via a clustering algorithm in a training set to obtain a plurality of subtypes stably appearing in the training set and a marker gene for each subtype; conducting enrichment analysis on marker genes for the plurality of subtypes, conducting immune cell infiltration evaluation on the plurality of subtypes, and dividing the plurality of subtypes into a plurality of subtype classes according to results of the analysis and the evaluation; comparing treatment response rates of different subtype classes through a comparison set to determine a subtype class to be identified; constructing a support vector machine model with feature genes screened and an optimal parameter combination; and determining whether immune-related disease data to be classified is the subtype class to be identified.

Abstract: A method and a system of molecular typing and subtyping classifier for immune-related diseases are provided. The method includes: conducting molecular typing via a clustering algorithm in a training set to obtain a plurality of subtypes stably appearing in the training set and a marker gene for each subtype; conducting enrichment analysis on marker genes for the plurality of subtypes, conducting immune cell infiltration evaluation on the plurality of subtypes, and dividing the plurality of subtypes into a plurality of subtype classes according to results of the analysis and the evaluation; comparing treatment response rates of different subtype classes through a comparison set to determine a subtype class to be identified; constructing a support vector machine model with feature genes screened and an optimal parameter combination; and determining whether immune-related disease data to be classified is the subtype class to be identified.

Abstract: The present invention provides a general sequence of chimeric antigen receptor (CAR) and an application thereof, The hinge domain in the general sequence includes hinge domain V-5, hinge domain V-4-5, hinge domain V-3-4-5, hinge domain V-2-3-4-5 or hinge domain V-1-2-3-4-5. The CAR constructed by using the general sequence of the chimeric antigen receptor of the present invention can significantly improve the antigen-specific immune response of CAR-T cells, enhance the sensitivity of CAR-T cells and resist exhaustion, so as to enhance the therapeutic effect of CAR-T cells and enhance the therapeutic effect of anti-tumor agents. At the same time, the CAR containing the general sequence of the present invention is suitable for transfection of immune cells such as T cells and NK cells, and can be broadly applied to tumor therapy.

Abstract: The present invention provides a general sequence of chimeric antigen receptor (CAR) and an application thereof, The hinge domain in the general sequence includes hinge domain V-5, hinge domain V-4-5, hinge domain V-3-4-5, hinge domain V-2-3-4-5 or hinge domain V-1-2-3-4-5. The CAR constructed by using the general sequence of the chimeric antigen receptor of the present invention can significantly improve the antigen-specific immune response of CAR-T cells, enhance the sensitivity of CAR-T cells and resist exhaustion, so as to enhance the therapeutic effect of CAR-T cells and enhance the therapeutic effect of anti-tumor agents. At the same time, the CAR containing the general sequence of the present invention is suitable for transfection of immune cells such as T cells and NK cells, and can be broadly applied to tumor therapy.