Abstract: A combined HER2 and MESO dual-target CAR-T vector, a construction method therefor and an application thereof in cancer are provided; specifically, a bispecific chimeric antigen receptor (CAR) containing HER2 scFv and MESO scFv, a 4-1BB co-stimulatory domain, and a CD3 activation domain is provided. The bispecific CAR-T cell has a significant killing effect on HER2-positive target cells and MESO-positive target cells and can improve on the tumor-killing effect of T cells; the cytokines produced have a super-additive effect, and compared with a single-target CAR-T, can better clear tumor cells, alleviate antigen escape caused by tumor heterogeneity, and further strengthen the ability of CAR-T cells to kill tumors.

Abstract: An audio processing method is provided. The method includes: obtaining to-be-processed audio acquired by an audio acquisition end; performing filtering processing on the to-be-processed audio to obtain a processing result, wherein the filtering processing is used for filtering out partial audio signal components from the to-be-processed audio, and frequencies of the partial audio signal components are lower than a preset threshold; extracting a plurality of speech frames within a first preset duration from the processing result; obtaining an energy variation amount of the plurality of speech frames; and determining a category of the to-be-processed audio based on the energy variation amount.

Abstract: A natural edible shampoo and preparation method thereof, the components comprises fatty acyl lactyl-lactic acid, arginine, vegetable oleic acid monoglyceride, plant phospholipid, ?-polylysine, ethyl lauroyl arginate hydrochloride, inulin, polyol and so on. It resist static electricity, improve the smoothness of hair, prevent pore clogging, and has good flowability at a low temperature. The shampoo overturns the classic concept of existing cationic hair care (large irritation), uses nearly non-irritant natural anionic wash and care main ingredients, relies on a non-enclosed protein peptide/low HLB value oiliness agent film-forming smoothing system, avoids a cationic polymer/silicone oil hair-care pattern with intensive negative effect, and protects the original ecological environment of hair and scalp, so that a final product gains the smooth experience feeling of organic silicone oil, thereby creating a new path for research and development of food and cosmetic-type wash and care two-in-one shampoos.