Abstract: The present invention provides high-complexity defined gut microbial communities capable of achieving substantial engraftment and having stability following human fecal community microbial challenge and methods of producing the same. Also provided are methods of using high-complexity defined gut microbial communities for the treatment of dysbiosis or a pathological condition in an animal.

Abstract: Methods for characterizing a population of microbes in a sample are described. The methods include: amplifying microbial polynucleotides obtained from the sample to form a plurality of amplicons and combining the amplicons with a plurality of microbeads, wherein each of the microbeads has a lanthanide spectral signature paired with the sequence of capture polynucleotides immobilized on the microbead. At least some of the capture polynucleotides comprise a sequence substantially complementary to a microbe-identifying sequence in one or more amplicons, such that at least some amplicons are captured onto beads by the capture polynucleotides and the microbe can be identified based on the lanthanide spectral signature of with which the capture polynucleotide is paired. Further described are methods for the identification of pathogens present in a sample by evaluating patterns of hybridization of a capture oligonucleotide to amplicons.

Abstract: The present invention provides high-complexity defined gut microbial communities capable of achieving substantial engraftment and having stability following human fecal community microbial challenge and methods of producing the same. Also provided are methods of using high-complexity defined gut microbial communities for the treatment of dysbiosis or a pathological condition in an animal.

Abstract: The disclosure provides Cas9 protein variants that are thermostable at elevated temperatures (e.g., at least 70° C. or above). A Cas9 protein may have at least 75% sequence identity to a wild-type Cas9 protein (e.g., a wild-type Cas9 protein having the sequence of SEQ ID NO:1) and/or one or more amino acid substitutions relative to the wild-type Cas9 protein.

Abstract: A computing device configured to communicate with an input device. The computing device includes a processor, a touch interface, such as a touch screen, and a receiving unit. The touch interface is configured to detect an input signal corresponding to an object approaching or contacting a surface. The receiving unit is configured to receive, through the touch interface, at least one input signal from the input device, and the receiving unit amplifies the at least one input signal creating at least one amplified input signal. Additionally, at least one of the processor or the receiving unit analyzes the at least one amplified input signal and creates at least one output digital signal corresponding to the at least one input signal.

Abstract: A computing device configured to communicate with an input device. The computing device includes a processor, a touch interface, such as a touch screen, and a receiving unit. The touch interface is configured to detect an input signal corresponding to an object approaching or contacting a surface. The receiving unit is configured to receive, through the touch interface, at least one input signal from the input device, and the receiving unit amplifies the at least one input signal creating at least one amplified input signal. Additionally, at least one of the processor or the receiving unit analyzes the at least one amplified input signal and creates at least one output digital signal corresponding to the at least one input signal.