Abstract: A method of preparing a reconstitutable implantable bone putty includes combining a bone matrix derived from human bone and gelatin particulates derived from human tissue at a concentration of the bone matrix by dry weight of 20 to 60 percent to form the reconstitutable implantable bone putty. Preparing the gelatin particulates includes supplying a gelatin precursor of bone or soft tissue from a human, treating the gelatin precursor with phosphoric acid to generate a gelatin-acid mixture, neutralizing the gelatin-acid mixture with an alkali to a pH between 6 and 8 to allow a gelatin-rich solution and a waste solution to separate, removing residual salts from the gelatin-rich solution to obtain purified gelatin, drying the purified gelatin, and reducing the purified gelatin to particulates having a largest dimension less than 300 ?m. A method of preparing an implantable bone putty includes adding a reconstitution media to the reconstitutable implantable bone putty.

Abstract: A method of preparing a reconstitutable implantable bone putty includes combining a bone matrix derived from human bone and gelatin particulates derived from human tissue at a concentration of the bone matrix by dry weight of 20 to 60 percent to form the reconstitutable implantable bone putty. Preparing the gelatin particulates includes supplying a gelatin precursor of bone or soft tissue from a human, treating the gelatin precursor with phosphoric acid to generate a gelatin-acid mixture, neutralizing the gelatin-acid mixture with an alkali to a pH between 6 and 8 to allow a gelatin-rich solution and a waste solution to separate, removing residual salts from the gelatin-rich solution to obtain purified gelatin, drying the purified gelatin, and reducing the purified gelatin to particulates having a largest dimension less than 300 ?m. A method of preparing an implantable bone putty includes adding a reconstitution media to the reconstitutable implantable bone putty.

Abstract: Various embodiments describe methods of predicting an amount of a target osteoactivity marker. The methods include measuring an initial concentration of the target osteoactivity marker at a time t0; measuring an initial concentration of at least one additional osteoactivity marker at the time t0; and calculating a second concentration of the target osteoactivity marker at a time t1 based on the initial concentration of the target osteoactivity marker at the time t0 and the initial concentration of the at least one additional osteoactivity marker at the time t0.

Abstract: According to various embodiments, a bone product includes bone having at least one patterned surface. The patterned surface includes a plurality of pits. Each of the plurality of pits has a diameter of from about 5 ?m to about 15 ?m and a depth of approximately half of the diameter.