Abstract: An apparatus for reducing a chromatic spread angle of light diffracted at an acousto-optic element includes the acousto-optic element and a first and a second focusing optical unit. The acousto-optic element is disposed in a beam path of an incident light beam and is configured to generate the diffracted light from the incident light beam such that the diffracted light emanates from a virtual interaction point of the acousto-optic element. The first focusing optical unit is disposed in the beam path upstream of the acousto-optic element and the second focusing optical unit is disposed in the diffracted light such that a focus of the incident light beam is situated downstream of the first focusing optical unit in the acousto-optic element and the virtual interaction point is located in a front focus of the second focusing optical unit.

Abstract: A scanning microscope includes an objective arranged in an illuminating beam path and configured to focus an illuminating light bundle onto a sample. A scanning unit is arranged upstream of the objective in the illuminating beam path and configured to deflect the illuminating light bundle in such a way that the illuminating light bundle focused by the objective executes a scanning movement. A detection unit is arranged in a detection beam path and configured to receive a detection light bundle not deflected by the scanning unit. For spectral influencing of the detection light bundle, the detection unit contains a spectrally selective component which has an active surface with a spectral edge which varies with a location of incidence of the detection light bundle on the active surface. The active surface is arranged in the detection beam path at a location of the image of the objective pupil.

Abstract: A method for illuminating a sample slice uses a light beam or a light sheet during single plane illumination microscopy (SPIM). The light beam or light sheet is deflected by an angle mirror having a first and second reflective surface reflecting a first and second portion of the light beam or light sheet, respectively, whereby the first and second portions of the light beam or light sheet spatially overlap one another after the deflecting. Alternatively, the light beam or light sheet is refracted by a refractive optical component comprising a first and second refractor surface refracting a first portion of the light beam or light sheet, respectively, whereby the first and second portions of the light beam or light sheet spatially overlap one another after the refracting.

Abstract: An apparatus for reducing a chromatic spread angle of light diffracted at an acousto-optic element includes the acousto-optic element and a first and a second focusing optical unit. The acousto-optic element is disposed in a beam path of an incident light beam and is configured to generate the diffracted light from the incident light beam such that the diffracted light emanates from a virtual interaction point of the acousto-optic element. The first focusing optical unit is disposed in the beam path upstream of the acousto-optic element and the second focusing optical unit is disposed in the diffracted light such that a focus of the incident light beam is situated downstream of the first focusing optical unit in the acousto-optic element and the virtual interaction point is located in a front focus of the second focusing optical unit.

Abstract: A scanning microscope includes an objective arranged in an illuminating beam path and configured to focus an illuminating light bundle onto a sample. A scanning unit is arranged upstream of the objective in the illuminating beam path and configured to deflect the illuminating light bundle in such a way that the illuminating light bundle focused by the objective executes a scanning movement. A detection unit is arranged in a detection beam path and configured to receive a detection light bundle not deflected by the scanning unit. For spectral influencing of the detection light bundle, the detection unit contains a spectrally selective component which has an active surface with a spectral edge which varies with a location of incidence of the detection light bundle on the active surface. The active surface is arranged in the detection beam path at a location of the image of the objective pupil.

Abstract: A scanning microscope includes an objective arranged in an illuminating beam path to focus an illuminating light bundle onto a sample. A scanning unit is arranged upstream of the objective to deflect the illuminating light bundle such that it is focused by the objective executes a scanning movement on the sample. A detection unit is arranged in a detection beam path to receive a detection light bundle not deflected by the scanning unit. For spectral influencing of the detection light bundle, the detection unit contains a spectrally selective component which has an active surface with a spectral edge which varies with the location of incidence of the detection light bundle on the active surface. The active surface is arranged in the detection beam path at the location of an image of an objective pupil, or in a position at which a variation of the spectral edge is compensated for.

Abstract: A method for illuminating a sample slice uses a light beam or a light sheet during single plane illumination microscopy (SPIM). The light beam or light sheet is deflected by an angle mirror having a first and second reflective surface reflecting a first and second portion of the light beam or light sheet, respectively, whereby the first and second portions of the light beam or light sheet spatially overlap one another after the deflecting. Alternatively, the light beam or light sheet is refracted by a refractive optical component comprising a first and second refractor surface refracting a first portion of the light beam or light sheet, respectively, whereby the first and second portions of the light beam or light sheet spatially overlap one another after the refracting.

Abstract: A scanning microscope includes an objective arranged in an illuminating beam path to focus an illuminating light bundle onto a sample. A scanning unit is arranged upstream of the objective to deflect the illuminating light bundle such that it is focused by the objective executes a scanning movement on the sample. A detection unit is arranged in a detection beam path to receive a detection light bundle not deflected by the scanning unit. For spectral influencing of the detection light bundle, the detection unit contains a spectrally selective component which has an active surface with a spectral edge which varies with the location of incidence of the detection light bundle on the active surface. The active surface is arranged in the detection beam path at the location of an image of an objective pupil, or in a position at which a variation of the spectral edge is compensated for.