Abstract: A method of manipulating and/or investigating cellular bodies (9) is provided. The method comprises the steps of: providing a sample holder (3) comprising a holding space (5) for holding a fluid medium (11); providing a sample (7) comprising one or more cellular bodies (9) in a fluid medium (11) in the holding space (5); generating an acoustic wave in the holding space exerting a force (F) on the sample (7) in the holding space (5). The method further comprises providing the holding space (5) with a functionalised wall surface portion (17) to be contacted by the sample (7) and the sample (7) is in contact with the functionalised wall surface portion (17) during at least part of the step of application of the acoustic wave. A system and a sample holder (3) are also provided.

Abstract: A method is disclosed for characterizing an acoustic-based particle manipulation device comprising a sample holder having a holding space containing a fluid medium. The holding space comprises a wall surface portion. The method comprises providing a driving signal to the sample holder for generating an acoustic wave in the holding space that drives a particle at the wall surface portion, away from the wall surface portion. The method further comprises measuring vibrations of at least part of a surface of the sample holder caused by said driving signal. The method also comprises, based on the measured vibrations of said at least part of the surface, determining, for each position out of a plurality of positions at the wall surface portion and in the holding space, a value for an acoustic force that is caused by said driving signal and that acts in a direction away from the wall surface portion.

Abstract: A method of manipulating and/or investigating cellular bodies (9) is provided. The method comprises the steps of: providing a sample holder (3) comprising a holding space (5) for holding a fluid medium (11); providing a sample (7) comprising one or more cellular bodies (9) in a fluid medium (11) in the holding space (5); generating an acoustic wave in the holding space exerting a force (F) on the sample (7) in the holding space (5). The method further comprises providing the holding space (5) with a functionalised wall surface portion (17) to be contacted by the sample (7) and the sample (7) is in contact with the functionalised wall surface portion (17) during at least part of the step of application of the acoustic wave. A system and a sample holder (3) are also provided.

Abstract: A method is disclosed for measuring a temperature of a fluid in a holding space of an acoustic-based particle manipulation device. The method comprises performing a calibration. The calibration comprises measuring, using a reference temperature sensor, the temperature of the fluid to be a reference temperature value. The calibration also comprises determining a reference resonance frequency of the acoustic-based particle manipulation device having the fluid in its holding space at the reference temperature value. The method further comprises measuring the temperature of the fluid comprising determining a resonance frequency of the acoustic-based particle manipulation device and determining, based on the reference resonance frequency determined during the calibration and based on the determined resonance frequency, the temperature of the fluid to be a temperature value.

Abstract: The disclosure relates to methods and systems for manipulating a particle, in particular for studying small particles and/or biological cellular bodies. A disclosed method comprises the steps of: providing a sample holder (3) comprising a holding space (5) for holding a sample comprising a particle (9) in a fluid medium (11); providing a driving signal, having a signal frequency, a signal amplitude and a signal power, to the sample holder generating an acoustic wave in the sample holder (3) for applying an acoustic force to the particle (9), the wave having a frequency; determining an acoustic resonance frequency of the sample holder and determining a quality factor for the sample holder with respect to the resonance frequency. An associated system (1) is also disclosed.

Abstract: Systems and methods for manipulating and/or investigating objects, in particular biological objects such as cellular bodies, in a sample holder are provided. The sample holder comprises a holding space for holding a sample comprising one or more objects in a fluid medium. An acoustic wave generator is connected with the sample holder to generate an acoustic wave in the holding space exerting a force on at least part of the sample. Fluid flows through microfluidic channels in the sample holder and acoustic waves are controlled.

Abstract: A method comprises providing a sample holder having a holding space for a sample comprising particle(s) in a fluid medium, the holder comprising a wall providing a wall surface portion, and providing a signal generator for generating an acoustic wave in the holder; providing using the signal generator a driving signal to the holder generating a standing longitudinal acoustic wave in the holder comprising at least one of a node and an antinode; and determining an acoustic resonance frequency characteristic. The method further comprises: providing a sample comprising particle(s) in a fluid medium in contact with the wall surface portion; determining a variation in the resonance frequency characteristic of the holder; determining a difference in position of one or more of the particles with respect to a node and/or an antinode of the acoustic wave and/or the wall surface portion, in particular contact, attachment and/or adhesion.

Abstract: A method of manipulating and/or investigating cellular bodies (9) is provided. The method comprises the steps of: providing a sample holder (3) comprising a holding space (5) for holding a fluid medium (11); providing a sample (7) comprising one or more cellular bodies (9) in a fluid medium (11) in the holding space (5); generating an acoustic wave in the holding space exerting a force (F) on the sample (7) in the holding space (5). The method further comprises providing the holding space (5) with a functionalised wall surface portion (17) to be contacted by the sample (7) and the sample (7) is in contact with the functionalised wall surface portion (17) during at least part of the step of application of the acoustic wave. A system and a sample holder (3) are also provided.

Abstract: A method of manipulating and/or investigating cellular bodies (9) is provided. The method comprises the steps of: providing a sample holder (3) comprising a holding space (5) for holding a fluid medium (11); providing a sample (7) comprising one or more cellular bodies (9) in a fluid medium (11) in the holding space (5); generating an acoustic wave in the holding space exerting a force (F) on the sample (7) in the holding space (5). The method further comprises providing the holding space (5) with a functionalised wall surface portion (17) to be contacted by the sample (7) and the sample (7) is in contact with the functionalised wall surface portion (17) during at least part of the step of application of the acoustic wave. A system and a sample holder (3) are also provided.

Abstract: A method of manipulating and/or investigating cellular bodies (9) is provided. The method comprises the steps of: providing a sample holder (3) comprising a holding space (5) for holding a fluid medium (11); providing a sample (7) comprising one or more cellular bodies (9) in a fluid medium (11) in the holding space (5); generating an acoustic wave in the holding space exerting a force (F) on the sample (7) in the holding space (5). The method further comprises providing the holding space (5) with a functionalised wall surface portion (17) to be contacted by the sample (7) and the sample (7) is in contact with the functionalised wall surface portion (17) during at least part of the step of application of the acoustic wave. A system and a sample holder (3) are also provided.