Abstract: The invention belongs to the field of genetic engineering technology and application thereof, and in order to solve the problems of lack of high efficient TMV-resistant RNAi nano-preparation at present, complicated preparation process of medicament, poor stability and delivery efficiency of dsRNA, the invention provides an RNAi nano-preparation, preparation method thereof and application thereof in TMV prevention and control. The RNAi nano-preparation is prepared from dsRNA and chitosan nano materials, wherein, dsRNA is a highly TMV-resistant RdRP3 gene with a length of 313 bp obtained from screening, with nucleotide sequence shown in SEQ ID NO.1, and the volume ratio of the chitosan to 1 ?g/?l dsRNA is 10:(1-6).

Abstract: The present invention belongs to the technical field of application of microbial secondary metabolites, and particularly relates to novel use of prodigiosin in resisting potato virus Y. Application of prodigiosin in preparing a medicine for preventing and controlling potato virus Y. The medicine for preventing and controlling the potyvirus is a liquid or solid preparation containing prodigiosin. It is clarified that PVY promotes its own replication and infection by recruiting host factor Hsp70 in the process of infecting the host, realizing virus infection and spread. Hsp70 plays an important role in the infection and replication of plant viruses. Based on the above results, it is further clarified that prodigiosin treatment can significantly increase the ubiquitination level of the plant host, promote the ubiquitination of HSP70 protein of the host, degrade or inhibit the expression of Hsp70 protein, activate the natural immune activity of the host plant.

Abstract: The present invention belongs to the technical field of application of microbial secondary metabolites, and particularly relates to novel use of prodigiosin in resisting potato virus Y. Application of prodigiosin in preparing a medicine for preventing and controlling potato virus Y. The medicine for preventing and controlling the potyvirus is a liquid or solid preparation containing prodigiosin. It is clarified that PVY promotes its own replication and infection by recruiting host factor Hsp70 in the process of infecting the host, realizing virus infection and spread. Hsp70 plays an important role in the infection and replication of plant viruses. Based on the above results, it is further clarified that prodigiosin treatment can significantly increase the ubiquitination level of the plant host, promote the ubiquitination of HSP70 protein of the host, degrade or inhibit the expression of Hsp70 protein, activate the natural immune activity of the host plant.

Abstract: The invention belongs to the field of genetic engineering technology and application thereof, and in order to solve the problems of lack of high efficient TMV-resistant RNAi nano-preparation at present, complicated preparation process of medicament, poor stability and delivery efficiency of dsRNA, the invention provides an RNAi nano-preparation, preparation method thereof and application thereof in TMV prevention and control. The RNAi nano-preparation is prepared from dsRNA and chitosan nano materials, wherein, dsRNA is a highly TMV-resistant RdRP3 gene with a length of 313 bp obtained from screening, with nucleotide sequence shown in SEQ ID NO.1, and the volume ratio of the chitosan to 1 ?g/?l dsRNA is 10:(1-6).

Abstract: The disclosure discloses a solid material drying system, including a drying tower and a closed annular conveyor belt at the bottom of the drying tower. A solid material enters the drying tower through the conveyor belt, and a first airflow moves towards the top of the drying tower from the bottom of the drying tower through the conveyor belt. Side walls of the drying tower are provided with several openings, and a second airflow moves towards the outside of the drying tower from the inside of the drying tower. Blocking plates that can block the openings are disposed at positions close to and above the openings inside the drying tower, and the blocking plates are hinged with the side walls of the drying tower. The disclosure is suitable for drying solid materials, and can be efficiently and universally applied to the drying of various types of solid materials.

Abstract: The invention provides an application of a nano RNAi preparation in PVY prevention and control, belongs to the field of genetic engineering and application thereof, and can solve the problem of the nano RNAi preparation in PVY virus prevention and control. The RNAi preparation is prepared from dsRNA and chitosan nano materials, wherein the dsRNA is prepared from three gene capsid proteins CP, auxiliary components-protease HC-Pro and genome connexin VPg, and the dsRNA plays a key role in replication, proliferation and movement of PVY virus in plants. The nano RNAi preparation provided by the invention is used for virus prevention and control of PVY, so that the stability of dsRNA is stronger, the effect is longer, a good virus prevention and control effect can be achieved, and the nano RNAi preparation has a good application prospect in the field of PVY virus prevention and control.