Abstract: A heparin skeleton synthase originates from Neisseria animaloris, with an amino acid sequence as shown in SEQ ID NO.2 and a nucleotide sequence of the coding gene as shown in SEQ ID NO.1. Its recombinant expression level is 6.8 times that of the existing heparin skeleton synthase KfiA from Escherichia coli K5, and total enzyme activity per fermentation liquor is 5.22 times that of the heparin skeleton synthase KfiA. The heparin skeleton synthase mutants obtained through site-directed mutagenesis of the sites No. 16, No. 25, No. 30, No. 111, No. 165, and No. 172 in the amino acid sequence of the said heparin skeleton synthase all have high expression levels.

Abstract: A ventilation and heat dissipation apparatus of a wind-assisted rotor includes a cylinder (2), a top cover (3), and a rain shielding plate (4). The top cover (3) is disposed at the top of the cylinder (2) and covers the top of the cylinder (2), and the top cover (3) is provided with a manhole (1) communicating with an inner cavity of the cylinder (2). The rain shielding plate (4) is disposed above the manhole (1) and covers the manhole (1), and the rain shielding plate (4) and the top cover (3) are spaced apart to form a heat dissipation gap that communicates with an external atmosphere.