Patents by Inventor Fija M. Lagerwerf

Fija M. Lagerwerf has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Assay for the separation and quantification of hemagglutinin antigens
    Patent number: 8283452
    Abstract: Described are methods for separating hemagglutinin (HA) antigens, comprising the steps of applying a reduced and derivatized antigen preparation comprising solubilized HA antigens and a detergent in a pH controlled solution, on a Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) column; and eluting the HA antigens from the column with an ion pairing agent in an organic mobile phase. The invention further relates to quantifying methods using the methods for separating the antigens with the further step of measuring the peak area of the eluted antigen in a chromatogram resulting from the elution step.
    Type: Grant
    Filed: October 2, 2009
    Date of Patent: October 9, 2012
    Assignee: Crucell Holland B.V.
    Inventors: Johan C. Kapteyn, Fija M. Lagerwerf
  • Assay for the separation and quantification of hemagglutinin antigens
    Patent number: 7687611
    Abstract: The present invention relates to novel methods for separating hemagglutinin (HA) antigens, comprising the steps of applying a reduced and derivatized antigen preparation comprising solubilized HA antigens and a detergent in a pH controlled solution, on a Reversed-Phase High-Performance Liquid Chromatography column; and eluting the HA antigens from the column with an ion pairing agent in an organic mobile phase. The invention further relates to quantifying methods using the methods for separating the antigens with the further step of measuring the peak area of the eluted antigen in a chromatogram resulting from the elution step.
    Type: Grant
    Filed: May 2, 2005
    Date of Patent: March 30, 2010
    Assignee: Crucell Holland B.V.
    Inventors: Johan C. Kapteyn, Fija M. Lagerwerf