Abstract: N-terminal cardiac myosin-binding protein C (cMyBP-C) domains (C0-C2) bind to thick (myosin) and thin (actin) filaments to facilitate contraction and relaxation of the heart. These interactions are regulated by phosphorylation of the M-domain situated between domains C1 and C2. In cardiomyopathies and heart failure, phosphorylation of cMyBP-C is significantly altered. A current challenge is to understand myosin- and actin-C0-C2 interactions in the context of mutations and phosphorylation states. The combinatorial analysis needed is challenging with current low-throughput assays. Described herein are time-resolved fluorescence resonance energy transfer (TR-FRET) high-throughput assays to meet this need.

Abstract: A content delivery (CD) service in a CDN maintains property invalidation information on said CD service including, for each property, a list of one or more group invalidation commands. New group invalidation commands are added by selectively merging one or more invalidation commands to form a merged invalidation command and inserting the merged invalidation command into said list, wherein the merging is based on a length of common prefix of elements in said list. A per property common prefix list is maintained for invalidation commands in each property list.

Abstract: A content delivery (CD) service in a CDN maintains property invalidation information on said CD service including, for each property, a list of one or more group invalidation commands. New group invalidation commands are added by selectively merging one or more invalidation commands to form a merged invalidation command and inserting the merged invalidation command into said list, wherein the merging is based on a length of common prefix of elements in said list. A per property common prefix list is maintained for invalidation commands in each property list.