Abstract: Manual samplers and sampling methods that facilitate improved dexterity and ease of use and that can be manufacture quickly and cost effectively are provided herein. Such sampler can include sampling sheet(s) with a pocket defined by seams to accommodate a user's entire hand for manual sampling of one or more products, in particular aggregate sampling of multiple products. Samplers can include entry seams to fit along the user's writs to retain the sampler on the user's hand and/or interior seams that engage the user's digits to improve dexterity while contact sampling of uneven surfaces.

Abstract: Powered sampling systems, devices and methods of surface sampling are provided herein. A portable handheld sampling device can include an actuator operably coupled with a sampling head supporting a sample medium. One or more sensor indicate when a suitable force or pressure is applied by the sampling device as well as the duration of sampling. The actuator can provide movement of the sampling medium, such as rotational movement. The sampling device can provide guidance to a user to facilitate standardized sampling according to a sampling protocol. The sampling device and system can include communication means to facilitate automatic sample logging and period updates of sample protocols. System for creating and managing sampling plans and test results are also provided herein.

Abstract: Sampling probe devices, systems and methods for sampling frozen or solid meltable products. Such systems can include a heating element for placing against the frozen or solid product and a sampler with a cover fabricated at least partly from a microbial sampling medium so the cover absorbs a liquid sample from the product. Methods of sampling include heating the product and contacting the product with the cover until sufficient liquid sample is absorbed by the cover of the sampler. The cover can be weighed after sampling and compared to a before-sampling weight to confirm sufficient liquid was obtained. The sample can be tested as an aggregate sample that is representative of the lot or batch of product being sampled, including testing without an enrichment procedure. Systems utilizing automated heated-sampling plates and radiative heaters are also described.

Abstract: Sampling probe devices and methods for sampling frozen or solid meltable products are provided herein. Such devices can include a sampling probe with heating element and a cover fabricated at least partly from a microbial sampling medium that fits over the probe and heating element so that when the heated probe is placed against the frozen or solid product, the cover absorbs a liquid sample from the product. Methods of sampling include heating the probe and contacting the product with the cover until sufficient liquid sample is absorbed by the cover. The cover can be weighed after sampling and compared to a before-sampling weight to confirm sufficient liquid was obtained. The sample can be tested as an aggregate sample that is representative of the lot or batch of product being sampled, including testing without an enrichment procedure. Systems utilizing automated heated-sampling plates and radiative heaters are also described.

Abstract: Methods and devices for non-invasively assessing lethality and/or cross contamination of a process. In some embodiments, an aggregating sampler is used, such as a fixture catcher, to obtain samples before, after and/or during the process. In some embodiments, an isolated packet of bacteria is exposed to the active elements of the process without contacting the product. In other embodiments, a method to measure lethality using microgenomic analysis is reported. In still other embodiments, a procedure is reported to use the knowledge from a microgenomic process to use direct qPCR for identified genera species to measure cross contamination. These metrics have special utility in the validation of wash water performance but may have utility is assessing process performance when unpackaged product is treated as for blanching and irradiation. Process performance can include verification of process delivery or for research.

Abstract: Powered sampling systems, devices and methods of surface sampling are provided herein. A portable handheld sampling device can include an actuator operably coupled with a sampling head supporting a sample medium. One or more sensor indicate when a suitable force or pressure is applied by the sampling device as well as the duration of sampling. The actuator can provide movement of the sampling medium, such as rotational movement. The sampling device can provide guidance to a user to facilitate standardized sampling according to a sampling protocol. The sampling device and system can include communication means to facilitate automatic sample logging and period updates of sample protocols. System for creating and managing sampling plans and test results are also provided herein.

Abstract: Systems and methods for sampling within a conveyance system are provided herein, such systems and methods being particularly useful in batch sampling of food products for a targeted biological agent. In one aspect, the sampling system includes a conveyance system and one or more sampling devices positioned along a conveyance path such that at least one portion of the batch contacts a sampling medium of the one or more sampling devices. In another aspect, sampling devices are provided that allow a sampling member to be secured in a sampling position for batch testing and readily removed after sampling and tested. The systems, devices and methods herein provide improved sampling coverage of the entire batch and reduce waste and inefficiency as compared to conventional batch sampling methods.

Abstract: Systems and methods for sampling within a conveyance system are provided herein, such systems and methods being particularly useful in batch sampling of food products for a targeted biological agent. In one aspect, the sampling system includes a conveyance system and one or more sampling devices positioned along a conveyance path such that at least one portion of the batch contacts a sampling medium of the one or more sampling devices. In another aspect, sampling devices are provided that allow a sampling member to be secured in a sampling position for batch testing and readily removed after sampling and tested. The systems, devices and methods herein provide improved sampling coverage of the entire batch and reduce waste and inefficiency as compared to conventional batch sampling methods.

Abstract: The invention relates generally to improving the growth properties of cells in culture and more specifically to accumulating beneficial mutations in the genome of cells growing in culture. Methods are disclosed for isolating cells with improved growth properties for a number of different adverse cell culture conditions which develop during prolonged culture of cells.