Abstract: A method for determining a neural network for correcting optical aberrations includes determining one or more images that are at least partly related to an optical system or the design of an optical system. A neural network is determined on the basis of the determined one or more images in such a way that the determined neural network when applied to an image captured by the optical system outputs an image which has been corrected in relation to one or more optical aberrations.

Abstract: A method for manipulating at least one beam path in a microscope includes ascertaining a refractive index of a sample arranged in a sample volume and/or of an optical medium arranged in the sample volume. At least one microscope parameter is set in dependence on the ascertained refractive index for manipulating the beam path.

Abstract: In a method for the examination of a sample the sample is illuminated in a sample plane along a sample line with an illuminating light beam. The sample is acted upon by a depletion or switching light beam, which overlaps in the sample plane in an overlap region at least partially spatially with the illuminating light beam and which has at least one wavelength suitable for depletion of the sample. Part of fluorescent light emanating from the sample plane is detected as detection light. The fluorescent light originating from outside a first subregion and a second subregion is at least partially suppressed and not detected.

Abstract: An optical device for a microscope includes a reflector assembly including a beam entrance, at which a light beam can be coupled into the reflector assembly, including a beam exit, and including a beam path extending within the reflector assembly. The beam path begins in the reflector assembly at the beam entrance and passes the beam exit multiple times. On each passage of the beam path, partial beams are coupled out of the reflector assembly at the beam exit. The partial beams coupled out at the beam exit either extend in parallel and so as to be spaced apart from one another or are directed out of the reflector assembly starting from a common starting point.

Abstract: An optical apparatus for production of light sheets includes an optical splitter system which, at an optical splitter system output, includes a plurality of collimated light beams converging on a common point; a retroreflector, including a beam input at which the plurality of light beams are coupled into the retroreflector, and a beam output at which the plurality of light beams are emitted from the retroreflector; and a light sheet optical system into which the plurality of light beams emitted from the beam output of the retroreflector extend. At a light sheet optical system output, a differently oriented light sheet is formed from each light beam. A path length covered within the retroreflector by the plurality of light beams between the beam input and the beam output is dependent on an angle of incidence of each light beam at the beam input.

Abstract: A light sheet fluorescence microscope includes a light source configured to emit excitation light suitable for inducing fluorescent light emitted from a specimen, a detector configured to detect the fluorescent light from the specimen, and an optical system configured to illuminate the specimen with a light sheet formed from the excitation light, and to guide the fluorescent light from the illuminated specimen to the detector. The optical system includes an objective facing the specimen, the objective being configured to collect the fluorescent light emitted from the specimen. The light source is further configured to emit manipulation light suitable for photomanipulating the specimen. The optical system is further configured to direct the manipulation light through a spatially limited sub-area of an entrance pupil of the objective onto the specimen along a light propagation direction which is different from a light propagation direction of the light sheet.

Abstract: A light sheet microscope, such as an oblique plane microscope or a swept confocally-aligned planar excitation (SCAPE) microscope, includes an optical system with a first optical element, a second optical element and a mirror assembly. In a first operational state of the microscope, the optical system is configured to transmit illumination light along a first illumination light path through the first optical element into a first sample volume, and configured to transmit observation light along a first observation light path from the first sample volume to a detector device. In a second operational state of the microscope, the optical system is configured to transmit the illumination light along a second illumination light path via the mirror assembly through the second optical element into a second sample volume, and configured to transmit observation light along a second observation light path from the second sample volume to the detector device.

Abstract: A light microscope includes a scan illumination unit, which is designed to illuminate a specimen having a line focus produced by an illumination light beam and moved transversely to a light propagation direction. A descanned detection unit is designed to produce a stationary first line image of a target region from detection light that originates from a target region of the specimen illuminated with the moving line focus. The scan illumination unit and the descanned detection unit have a common objective, which is designed to receive both the illumination light beam and the detection light. The descanned detection unit contains a dispersive element, which is designed to spectrally split the detection light in order to generate multiple second line images, corresponding to the first line image, with different spectral compositions.

Abstract: An inclined plane microscope includes a detection assembly for collecting scattered light and/or fluorescent light from regions of a sample volume illuminated by a light sheet, wherein the detection assembly, on its side facing away from the sample volume, is configured to image a real intermediate image of the regions of the sample illuminated by the light sheet, and wherein the real intermediate image is tilted in relation to a focal plane of the detection assembly, and an optical alignment unit for imaging a predetermined image field of the real intermediate image onto a detector, wherein the alignment unit has a depth of field region, wherein a focal plane of the alignment unit is tilted in relation to the real intermediate image, and wherein the predetermined image field of the real intermediate image to be imaged by the alignment unit is located completely within the depth of field region.

Abstract: An optical arrangement for an inclined-plane microscope, includes: an optical illumination and detection arrangement, an optical erecting unit for imaging the real intermediate image on a detector, wherein an optical axis of the erecting unit is inclined with respect to an optical axis of the optical illumination and detection arrangement and is oriented substantially perpendicularly to the real intermediate image plane, and an attachment element arranged on the erecting unit, extending in a direction of the real intermediate image, and forming an interface which is oriented substantially parallel to the real intermediate image plane, wherein beam paths of the illumination light and of the scattered and/or fluorescent light transmitted by the illumination and detection arrangement are coupled in and intersect on the image side of the illumination and detection arrangement, and wherein the beam path of the illumination light is spaced apart from the interface.

Abstract: A microscope system includes a microscope stage having a top surface configured to have a sample carrier arranged thereon, the sample carrier being configured to receive at least one sample. The microscope system also includes an imaging system configured to image the at least one sample. The microscope system also includes an injector configured to inject a predetermined amount of a liquid into the sample carrier by injecting multiple successive and temporally spaced jets of the liquid into the sample carrier, each jet including a predetermined portion of the amount of liquid.

Abstract: A light sheet microscope includes an object slide, and optical illumination and detection systems. The optical illumination system includes an illumination objective configured to illuminate a first object plane that is oblique relative to a slide plane with a light sheet. The optical detection system includes a detection objective and an image sensor device. The image sensor device is configured to define a first image plane which is orthogonal to an optical axis of the detection objective and to define a second image plane which is tilted relative to the first image plane. The detection objective is configured to image a focal plane onto the first image plane and to image a second object plane of the object onto the second image plane, the focal plane being coincident with the first object plane, and the second object plane being parallel to or coincident with the slide plane.

Abstract: A processor for demixing a fluorescent-light input signal of a fluorescence microscope, the fluorescent-light input signal including at least two fluorescence emission responses that overlap in time, each of the at least two fluorescence emission responses being representative of an individual impulse response of a fluorophore to a fluorescence-triggering light pulse of a clocked time series of fluorescence-triggering light pulses, the processor: receiving a trigger signal comprising a time series of time markers, the trigger signal being representative of a clocking rate, at which the clocked time series of fluorescence-triggering light pulses is generated; and separating at least one fluorescence emission response from the fluorescent-light input signal.

Abstract: A method for adjusting a focus of an optical system includes focusing measurement light in a sample space using an optical arrangement. The measurement light is transmitted on a sample side of the optical arrangement through at least one optical medium. The measurement light reflected by a reflector and transmitted through a further optical arrangement is detected using a detector arrangement. A working distance between the optical arrangement and the reflector is ascertained based on the measurement light detected by the detector, wherein a focus of the measurement light lies on the reflector for the working distance.

Abstract: A illumination arrangement for a microscope for illuminating a sample with a light sheet includes an illumination input configured to feed an illumination beam along an optical axis of the illumination arrangement and an illumination output which faces a sample side and is configured to output the illumination beam to the sample side. A focusing optical system is provided with a set depth of focus. At least one optical modification element is configured to geometrically modify the illumination beam. Different rays of the illumination beam intersect the optical axis within an axis intersection region at the illumination output. The axis intersection region extends over at least the depth of focus of the focusing optical system along the optical axis.

Abstract: A control device for controlling a microscope includes: a computation module. The control device: adjusts a sample volume velocity of a sample volume of the microscope relative to the microscope; and adjusts a light sheet velocity of the light sheet of the microscope relative to the microscope based on the sample volume velocity.

Abstract: A light sheet microscope includes an illuminator having a beam source which is designed to direct an illumination beam propagating along an illumination axis onto a sample. A light-sheet generator is designed to generate a light-sheet-like illumination light distribution illuminating the sample in a partial area from the illumination beam. A detection unit has a detector which is designed to capture detection light originating from the partial area of the sample illuminated with the illumination light distribution. The illuminator comprises a beam modulator adapted to modulate the illumination beam along the illumination axis in such a way that light exposure of the partial area of the sample illuminated by the illumination light distribution varies along the illumination axis.

Abstract: A microscope, which is a confocal microscope converted into a light sheet microscope, includes a microscope body and a mechanical receiving apparatus for microscope objectives, through which a microscope beam path extends. An optical module attachable to the receiving apparatus is configured to illuminate a sample volume and collect and transmit light from the sample volume. The optical module comprises: first and second optical arrangements with first and second beam paths that intersect in the sample volume, an optical beam path selector configured to combine the first and/or second beam path with the microscope beam path, and an attachment element arranged between the first or second optical arrangement and the sample volume, wherein the first or second beam path extend at least in sections through the attachment element in order to generate a light sheet. An area sensor is configured to detect light collected from the sample volume.

Abstract: A microscope system includes a light sheet microscopy functional unit and at least one further light microscopy functional unit. The light sheet microscopy functional unit has an illumination objective which is formed by a first objective and a detection objective which is formed by a separate second objective. The at least one further light microscopy functional unit has a detection objective that is formed by the second objective.

Abstract: An illumination arrangement for a microscope includes an illumination input configured to inject an illumination beam bundle and an illumination output configured to output at least two partial beam bundles generated from the illumination beam bundle. At least one diffractive optical element is configured to split the illumination beam bundle into the at least two partial beam bundles that propagate along partial beam paths, and is configured to effect a relative change in respective propagation directions of the at least two partial beam bundles with respect to one another, such that the at least two partial beam bundles output by the illumination arrangement are non-collinear with respect to one another at the illumination output.