Abstract: A computer-assisted method for determining a microfluidic circuit configured to reproduce a neuron circuit, and comprising including the following steps: —obtaining a description of the neuron circuit, the description of the neuron circuit comprising a plurality of neuron populations and at least one neuron connection; —determining at least one first parameter for each node of a plurality of nodes of the microfluidic circuit, each node being associated with and configured to receive one neuron population among the plurality of neuron populations of the neuron circuit; —determining at least one second parameter for at least one connection of the microfluidic circuit, each connection being associated with and configured to receive a neuron connection of the at least one neuron connection of the neuron circuit; —determining the positioning of each node of the plurality of nodes and of each connection of the at least one connection.

Abstract: A computer-assisted method for determining a microfluidic circuit configured to reproduce a neuron circuit, and comprising including the following steps: —obtaining a description of the neuron circuit, the description of the neuron circuit comprising a plurality of neuron populations and at least one neuron connection; —determining at least one first parameter for each node of a plurality of nodes of the microfluidic circuit, each node being associated with and configured to receive one neuron population among the plurality of neuron populations of the neuron circuit; —determining at least one second parameter for at least one connection of the microfluidic circuit, each connection being associated with and configured to receive a neuron connection of the at least one neuron connection of the neuron circuit; —determining the positioning of each node of the plurality of nodes and of each connection of the at least one connection.

Abstract: Method for producing a microfluidic device, the method comprising a step of producing a master mould, the master mould comprising a first support member and a second support member, the second support member comprising a substrate and microstructures, the substrate having a first surface and a second surface opposite the first surface, the step of producing the master mould comprising the following sub-steps:—producing the second support member by forming the microstructures on the first surface of the substrate;—3D printing the first support member using a 3D printer, with a printing resin, the dimensions of the first support member being coordinated with the dimensions of the substrate in order to hold the substrate;—inserting the substrate of the second support member into the first support member.