Abstract: Embodiments of the present invention generally relate to recombinant alpha 1-antitrypsin (AAT) proteins, including variants of human AAT with individually introduced mutations, compositions containing such recombinant AAT proteins and carriers, expression plasmids or vectors and host cells that express such recombinant AAT proteins, methods of producing such recombinant AAT proteins, and methods of treating AAT deficiency-related diseases, disorders, and conditions or diseases, disorders, and conditions resulting in protease-induced tissue damage in a subject in need thereof with the recombinant AAT proteins and/or recombinant AAT protein compositions described here. The recombinant AAT proteins derived from mammalian host cells as produced by the methods described here may be produced in large quantities, without any animal components, i.e., highly pure, highly glycosylated, and may be advantageously used over plasma-derived AAT.

Abstract: Recombinant proteins are of great commercial interest. Yet, most current production methods in mammalian cells involve the time- and labor-consuming step of creating stable cell lines. Production methods based on transient gene expression are advantageous in terms of speed and versatility, yet, thus far, those methods have not shown the specific productivity, batch yield and volumetric yield to be an economic alternative to stable cell lines. The inventors improved on the methodology of transient transfection and achieved commercially relevant yields in terms of specific productivity (exceeding 35 pg per cell per day), batch yield (exceeding 700 mg/l) and volumetric yield.

Abstract: Fluorescent protein expressing cells with very high fluorescence are described as well as a method to obtain such cells. The fluorescence of such cells can be as high as about 1000 times the auto-fluorescence of the respective host cell. Due to the high fluorescence, said cells are very suitable tools in the development, surveillance and/or optimization of production processes, for selection of culture media and process parameters and for the determination of cell growth and the detection, quantification and monitoring of biomass.

Abstract: Fluorescent protein expressing cells with very high fluorescence are described as well as a method to obtain such cells. The fluorescence of such cells can be as high as about 1000 times the auto-fluorescence of the respective host cell.