Abstract: A dental floss applicator has a receptacle for receiving a spool of dental floss therein and rewind and tension means associated with the receptacle and receptacle closure top for maintaining the dental floss under a certain degree of tension. A projecting holder from the receptacle top has two spaced tines extending therefrom for stretching a piece of clean dental floss therebetween. The structure is so designed that additional tension may be applied to the stretched piece of dental floss by the user's index finger while holding the applicator in one hand in an easy and comfortable manner.